MiR-17-5p and MKL-1 modulate stem cell characteristics of gastric cancer cells.
MiR-17-5p and MKL-1 modulate stem cell characteristics of gastric cancer cells.
复制标题
MiR-17-5p和MKL-1调节胃癌细胞的干细胞特征
DOI：
10.7150/ijbs.57338
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发表时间：
2021
影响因子：
9.2
通讯作者：
Liao XH
中科院分区：
文献类型：
--
作者：
Dai ZT;Xiang Y;Duan YY;Wang J;Li JP;Zhang HM;Cheng C;Wang Q;Zhang TC;Liao XH
Effectively targeting cancer stem cells to treat cancer has great therapeutic prospects. However, the effect of microRNA miR-17/MKL-1 on gastric cancer stem cells has not been studied yet. This study preliminarily explored the mechanism of miR-17/MKL-1 in gastric cancer stem cells. Many previous reports have indicated that microRNA and EMT regulated cancer stem cell characteristics, and miR-17 and MKL-1 were involved as a critical gene in migration and invasion in the EMT pathway. Through RT-PCR, Western Blot, flow cytometry, immunofluorescence, sphere formation xenograft tumor assays and drug resistance, the role of miR-17-5p and MKL-1 on promoting stem cell-like properties of gastric cancer were verified in vivo and vitro. Next, MKL-1 targets CD44, EpCAM, and miR -17-5p promoter verified by luciferase assay and ChIP. Besides, the TCGA database analysis found that both miR-17-5p and MKL-1 increased in gastric cancer, and the prognostic survival of the MKL-1 high expression group was reduced. It is found that MKL-1 promotes expression by targeting miR-17, CD44 and EpCAM promoters. Besides, the TCGA database analysis found that both miR-17-5p and MKL-1 increased in gastric cancer, and the prognostic survival of the MKL-1 high expression group was reduced. These findings reveal new regulatory signaling pathways for gastric cancer stem cells, thus it give new insights on potential early diagnosis and/or molecular therapy for gastric cancer.
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影响因子：
8
作者：
通讯作者：
--
影响因子：
--
作者：
Boix L;López-Oliva JM;Rhodes AC;Bruix J
通讯作者：
Bruix J
影响因子：
14
作者：
Jang, Eunji;Kim, Eunjung;Haam, Seungjoo
通讯作者：
Haam, Seungjoo
影响因子：
2.4
作者：
Fu Y;Du P;Zhao J;Hu C;Qin Y;Huang G
通讯作者：
Huang G
DOI：
10.1016/s0140-6736(09)60617-6
发表时间：
2009-08-08
期刊：
Lancet (London, England)
影响因子：
--
作者：
Hartgrink HH;Jansen EP;van Grieken NC;van de Velde CJ
通讯作者：
van de Velde CJ