Identification of a sex-linked SNP marker in the salmon louse (Lepeophtheirus salmonis) using RAD sequencing.
Identification of a sex-linked SNP marker in the salmon louse (Lepeophtheirus salmonis) using RAD sequencing.
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DOI：
10.1371/journal.pone.0077832
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sturm A
中科院分区：
文献类型：
--
作者：
Carmichael SN;Bekaert M;Taggart JB;Christie HR;Bassett DI;Bron JE;Skuce PJ;Gharbi K;Skern-Mauritzen R;Sturm A
鲑鱼虱（Lepeophtheirus salmonis (Krøyer, 1837)）是一种寄生桡足动物，如果不加以治疗，会对大西洋鲑鱼（Salmo salar Linnaeus, 1758）造成相当大的损害，并对大西洋鲑鱼养殖业造成重大损失。鲑鱼虱是雌雄同体的，通常性别比接近1:1。虽然这一观察结果表明，鲑鱼虱的性别决定是遗传的，只有很小的环境影响，但鲑鱼虱的性别决定机制尚不清楚。本文描述了一个性别连锁的单核苷酸多态性（SNP）标记的鉴定，为鲑鱼虱性别决定的遗传机制提供了第一个证据。采用限制性内切位点相关DNA测序（RAD-seq）技术从实验室培养的鲑鱼虱株中分离SNP标记。总计8500万个原始Illumina 100碱基对末端读取在24个不相关的个体中产生281,838个独特的rad标签。RAD标记Lsa101901与所有个体的表型性别完全相关，雌性为杂合子，雄性为纯合子。采用等位基因特异性PCR法进行基因分型，进一步在3个不相关的鲑鱼虱株中证实了这种SNP关联模式，在96个基因分型个体中显示出与表型性别的完全关联。标记Lsa101901位于prohibition -2基因的编码区，表现出性别依赖性的差异表达，通过RT-qPCR检测，成年雌鲑鱼虱的mRNA水平比成年雄鲑鱼虱高约1.8倍。本研究观察到的一种新的性别连锁SNP标记与鲑鱼虱的性别决定是一致的，是遗传的，遵循雌性杂合系统。标记Lsa101901为确定鲑鱼虱的遗传性别提供了一种工具，并可用于制定控制策略。
The salmon louse (Lepeophtheirus salmonis (Krøyer, 1837)) is a parasitic copepod that can, if untreated, cause considerable damage to Atlantic salmon (Salmo salar Linnaeus, 1758) and incurs significant costs to the Atlantic salmon mariculture industry. Salmon lice are gonochoristic and normally show sex ratios close to 1:1. While this observation suggests that sex determination in salmon lice is genetic, with only minor environmental influences, the mechanism of sex determination in the salmon louse is unknown. This paper describes the identification of a sex-linked Single Nucleotide Polymorphism (SNP) marker, providing the first evidence for a genetic mechanism of sex determination in the salmon louse. Restriction site-associated DNA sequencing (RAD-seq) was used to isolate SNP markers in a laboratory-maintained salmon louse strain. A total of 85 million raw Illumina 100 base paired-end reads produced 281,838 unique RAD-tags across 24 unrelated individuals. RAD marker Lsa101901 showed complete association with phenotypic sex for all individuals analysed, being heterozygous in females and homozygous in males. Using an allele-specific PCR assay for genotyping, this SNP association pattern was further confirmed for three unrelated salmon louse strains, displaying complete association with phenotypic sex in a total of 96 genotyped individuals. The marker Lsa101901 was located in the coding region of the prohibitin-2 gene, which showed a sex-dependent differential expression, with mRNA levels determined by RT-qPCR about 1.8-fold higher in adult female than adult male salmon lice. This study’s observations of a novel sex-linked SNP marker are consistent with sex determination in the salmon louse being genetic and following a female heterozygous system. Marker Lsa101901 provides a tool to determine the genetic sex of salmon lice, and could be useful in the development of control strategies.
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影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
影响因子：
3.7
作者：
Baxter SW;Davey JW;Johnston JS;Shelton AM;Heckel DG;Jiggins CD;Blaxter ML
通讯作者：
Blaxter ML
影响因子：
4.4
作者：
Houston RD;Davey JW;Bishop SC;Lowe NR;Mota-Velasco JC;Hamilton A;Guy DR;Tinch AE;Thomson ML;Blaxter ML;Gharbi K;Bron JE;Taggart JB
通讯作者：
Taggart JB
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
4.7
作者：
Bouchon, D;Rigaud, T;Juchault, P
通讯作者：
Juchault, P
影响因子：
2.5
作者：
Costello, Mark J.
通讯作者：
Costello, Mark J.