Mutation of the OsSAC1 Gene, which Encodes an Endoplasmic Reticulum Protein with an Unknown Function, Causes Sugar Accumulation in Rice Leaves
Mutation of the OsSAC1 Gene, which Encodes an Endoplasmic Reticulum Protein with an Unknown Function, Causes Sugar Accumulation in Rice Leaves
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OsSAC1 基因编码功能未知的内质网蛋白，其突变导致水稻叶片糖分积累
DOI：
10.1093/pcp/pcx203
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发表时间：
2018-03
影响因子：
4.9
通讯作者：
何光华
中科院分区：
文献类型：
--
作者：
朱小燕;沈文强;黄俊阳;张天泉;张孝波;崔元江;桑贤春;凌英华;李云峰;王楠;赵芳明;张长伟;杨正林;何光华
糖是植物产生的最丰富的有机化合物，可用于构建碳骨架并产生能量。糖积累 1 (OsSAC1) 基因编码一种功能未知的蛋白质，该蛋白质具有四个 N 端跨膜区和两个功能未知的保守结构域：DUF4220 和 DUF594。发现 OsSAC1 在幼叶底部和发育中的叶鞘中表达较差且特异。亚细胞定位结果表明，OsSAC1 与 ER:mCherry 共定位，并靶向内质网 (ER)。人们发现 OsSAC1 会影响水稻 (Oryza sativa) 中的糖分分配。 I2/KI 淀粉染色、超微结构观察和淀粉含量测量表明，ossac1 来源叶片中积累的淀粉颗粒比野生型 (WT) 来源叶片中积累的淀粉颗粒更多、更大。此外，ossac1 源叶中积累的蔗糖和葡萄糖浓度高于 WT 源叶中的蔗糖和葡萄糖浓度，而 ossac1 幼叶和发育中的叶鞘中观察到的蔗糖和葡萄糖浓度低于 WT 中的蔗糖和葡萄糖浓度。与 WT 源叶相比，ossac1 源叶中 OsAGPL1 和 OsAGPS1（负责淀粉合成）的表达量要高得多，OscFBP1、OscFBP2、OsSPS1 和 OsSPS11（负责蔗糖合成）以及 OsSWEET11、OsSWEET14 和 OsSUT1（负责蔗糖加载）的表达量显着减少。与野生型相比，在 ossac1 幼叶和发育中的叶鞘中检测到更多的水稻胞间连丝负调节因子 OsGSD1。这些结果表明，靶向 ER 的 OsSAC1 可能间接调节需碳的幼叶和发育中的叶鞘中的糖分配。
Sugars are the most abundant organic compounds produced by plants, and can be used to build carbon skeletons and generate energy. The sugar accumulation 1 (OsSAC1) gene encodes a protein with an unknown function that exhibits four N-terminal transmembrane regions and two conserved domains of unknown function, DUF4220 and DUF594. OsSAC1 was found to be poorly and specifically expressed at the bottoms of young leaves and in the developing leaf sheaths. Subcellular location results showed that OsSAC1 was co-localized with ER:mCherry and targeted the endoplasmic reticulum (ER). OsSAC1 has been found to affect sugar partitioning in rice (Oryza sativa). I2/KI starch staining, ultrastructure observations and starch content measurements indicated that more and larger starch granules accumulated in ossac1 source leaves than in wild-type (WT) source leaves. Additionally, higher sucrose and glucose concentrations accumulated in the ossac1 source leaves than in WT source leaves, whereas lower sucrose and glucose concentrations were observed in the ossac1 young leaves and developing leaf sheaths than in those of the WT. Much greater expression of OsAGPL1 and OsAGPS1 (responsible for starch synthesis) and significantly less expression of OscFBP1, OscFBP2, OsSPS1 and OsSPS11 (responsible for sucrose synthesis) and OsSWEET11, OsSWEET14 and OsSUT1 (responsible for sucrose loading) occurred in ossac1 source leaves than in WT source leaves. A greater amount of the rice plasmodesmatal negative regulator OsGSD1 was detected in ossac1 young leaves and developing leaf sheaths than in those of the WT. These results suggest that ER-targeted OsSAC1 may indirectly regulate sugar partitioning in carbon-demanding young leaves and developing leaf sheaths.
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影响因子：
7.4
作者：
Kötting, O;Pusch, K;Ritte, G
通讯作者：
Ritte, G
影响因子：
7.4
作者：
LIN, TP;CASPAR, T;PREISS, J
通讯作者：
PREISS, J
DOI：
10.1007/s00018-015-2052-6
发表时间：
2016-01
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Schwarz DS;Blower MD
通讯作者：
Blower MD
影响因子：
7.4
作者：
Srivastava, Avinash C.;Ganesan, Savita;Ayre, Brian G.
通讯作者：
Ayre, Brian G.
影响因子：
4.3
作者：
R. Häusler;Nils Helge Schlieben;U. Flügge
通讯作者：
R. Häusler;Nils Helge Schlieben;U. Flügge