Structure and dynamics of cholesterol-containing polyunsaturated lipid membranes studied by neutron diffraction and NMR.
Structure and dynamics of cholesterol-containing polyunsaturated lipid membranes studied by neutron diffraction and NMR.
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DOI：
10.1007/s00232-010-9326-6
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发表时间：
2011-01
影响因子：
2.4
通讯作者：
Gawrisch, Klaus
中科院分区：
文献类型：
--
作者：
Mihailescu, Mihaela;Soubias, Olivier;Worcester, David;White, Stephen H.;Gawrisch, Klaus
关键词：
A direct and quantitative analysis of the internal structure and dynamics of a polyunsaturated lipid bilayer composed of 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (18:0-22:6n3-PC) containing 29 mol% cholesterol was carried out by neutron diffraction, 2H-NMR and 13C-MAS NMR. Scattering length distribution functions of cholesterol segments as well as of the sn-1 and sn-2 hydrocarbon chains of 18:0-22:6n3-PC were obtained by conducting experiments with specifically deuterated cholesterol and lipids. Cholesterol orients parallel to the phospholipids, with the A-ring near the lipid glycerol and the terminal methyl groups 3 Å away from the bilayer center. Previously, we reported that the density of polyunsaturated docosahexaenoic acid (DHA, 22:6n3) chains was higher near the lipid–water interface. Addition of cholesterol partially redistributes DHA density from near the lipid–water interface to the center of the hydrocarbon region. Cholesterol raises chain-order parameters of both stearic acid and DHA chains. The fractional order increase for stearic acid methylene carbons C8–C18 is larger, reflecting the redistribution of DHA chain density toward the bilayer center. The correlation times of DHA chain isomerization are short and mostly unperturbed by the presence of cholesterol. The uneven distribution of saturated and polyunsaturated chain densities and the cholesterol-induced balancing of chain distributions may have important implications for the function and integrity of membrane receptors, such as rhodopsin.
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影响因子：
4.3
作者：
Soubias, O;Jolibois, F;Milon, A
通讯作者：
Milon, A
影响因子：
15
作者：
Feller, SE;Gawrisch, K;Woolf, TB
通讯作者：
Woolf, TB
影响因子：
2.9
作者：
Pitman, MC;Suits, F;Feller, SE
通讯作者：
Feller, SE
影响因子：
3.4
作者：
Bennett, Michael P.;Mitchell, Drake C.
通讯作者：
Mitchell, Drake C.
DOI：
10.1016/0005-2736(79)90284-0
发表时间：
1979-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MILJANICH, GP;SKLAR, LA;DRATZ, EA
通讯作者：
DRATZ, EA