Circulating Th1, Th2, Th9, Th17, Th22, and Treg Levels in Aortic Dissection Patients.
Circulating Th1, Th2, Th9, Th17, Th22, and Treg Levels in Aortic Dissection Patients.
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主动脉夹层患者的循环 Th1、Th2、Th9、Th17、Th22 和 Treg 水平
DOI：
10.1155/2018/5697149
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发表时间：
2018
影响因子：
4.6
通讯作者：
Lin Y
中科院分区：
文献类型：
--
作者：
Ye J;Wang Y;Wang Z;Ji Q;Huang Y;Zeng T;Hu H;Ye D;Wan J;Lin Y
背景研究表明，CD 4 + Th细胞亚群与动脉粥样硬化、高血压等血管疾病密切相关。本研究旨在探讨主动脉夹层（AD）患者外周血Th 1、Th 2、Th 9、Th 17、Th 22和Treg水平。方法采用流式细胞术、定量聚合酶链反应和酶联免疫吸附试验分别检测56例AD患者和24例非AD患者外周血中Th 1、Th 2、Th 9、Th 17、Th 22和Treg细胞及其转录因子和功能性细胞因子水平。此外，人主动脉血管平滑肌细胞（HASMCs）用生理盐水，血管紧张素II（Ang II），或血浆从AD患者。结果与NAD组相比，AD组Th 1、Th 9、Th 17、Th 22及其转录因子水平升高，Th 2、Treg及其转录因子水平呈下降趋势。此外，在AD患者中观察到较高的IFN-γ、IL-9、IL-17和IL-22水平以及较低的IL-4和IL-35水平。简单线性回归分析和二元Logistic回归分析显示，Th 1/IFN-γ、IL-9、Th 17/IL-17和Th 22/IL-22正调节AD的发生，而Th 2/IL-4和Treg/IL-35负调节AD的发生。AD患者血浆进一步增加Ang II处理的HASMCs的Bax mRNA水平，但降低Bcl 2和α-SMA mRNA水平。结论Th 1、Th 2、Th 9、Th 17、Th 22和Treg细胞活性的改变与AD的发病有关。不同的CD 4 + T细胞亚群在AD中发挥不同的作用。
Background Previous studies demonstrated that the subsets of CD4+ T helper (Th) cells are closely related to vascular diseases, including atherosclerosis and hypertension. This study is aimed at investigating the circulating Th1, Th2, Th9, Th17, Th22, and Treg levels in aortic dissection (AD) patients. Methods Blood samples from AD (n = 56) and non-AD (NAD, n = 24) patients were collected, and the circulating levels of Th1, Th2, Th9, Th17, Th22, and Treg cells and their transcription factors and functional cytokines were measured by flow cytometric analysis, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assays, respectively. In addition, the human aortic vascular smooth muscle cells (HASMCs) were treated with saline, angiotensin II (Ang II), or plasma from AD patients. Results Compared with the levels in the NAD group, the Th1, Th9, Th17, Th22, and their transcription factor levels were increased and the Th2, Treg, and their transcription factor levels exhibited a decreasing trend in AD patients. In addition, higher IFN-γ, IL-9, IL-17, and IL-22 levels and lower IL-4 and IL-35 levels were observed in AD patients. Simple linear regression analysis and binary logistic regression analysis suggested that Th1/IFN-γ, IL-9, Th17/IL-17, and Th22/IL-22 positively regulated the occurrence of AD, while Th2/IL-4 and Treg/IL-35 negatively regulated the occurrence of AD. Plasma from AD patients further increased Bax mRNA levels but decreased Bcl2 and α-SMA mRNA levels in Ang II-treated HASMCs. Conclusions Changes in Th1, Th2, Th9, Th17, Th22, and Treg activity are associated with the onset of AD. Different subsets of CD4+ T cells play different roles in the presence of AD.
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影响因子：
7.3
作者：
Duggleby R;Danby RD;Madrigal JA;Saudemont A
通讯作者：
Saudemont A
DOI：
10.2119/molmed.2014.00195
发表时间：
2015-01-01
期刊：
Molecular Medicine (Baltimore)
影响因子：
--
作者：
Meng, Kai;Zeng, Qiutang;Zhou, Yujie
通讯作者：
Zhou, Yujie
影响因子：
9.3
作者：
Madhumitha H;Mohan V;Deepa M;Babu S;Aravindhan V
通讯作者：
Aravindhan V
影响因子：
4.4
作者：
Erbel, Christian;Chen, Lili;Dengler, Thomas J.
通讯作者：
Dengler, Thomas J.
影响因子：
4.4
作者：
Soon，Megan S. F.;Haque，Ashraful
通讯作者：
Haque，Ashraful