In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.
In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.
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DOI：
10.1038/ncomms13475
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发表时间：
2016-11-15
影响因子：
16.6
通讯作者：
Dawson, Kenneth A.
中科院分区：
文献类型：
--
作者：
Lo Giudice, Maria Cristina;Herda, Luciana M.;Polo, Ester;Dawson, Kenneth A.
与活体相互作用或源自活体的纳米颗粒几乎总是被包裹在复杂生物环境中呈现的各种生物分子中，这些生物分子产生生物界面或生物分子日冕，赋予颗粒生物身份。纳米颗粒-生物分子复合体表面的生物分子呈现可被细胞受体或生物屏障识别的分子片段，可能与不同的生物途径相结合。在这里，我们证明了使用强烈的荧光报告绑定，在这种情况下，抗体与量子点结合，我们可以绘制出这种识别片段的可用性，从而允许快速和有意义的生物学特征。在微流体流中的应用，在较小的检测体积内，加上适当的检测阈值，可以在现实的生物环境中研究甚至复杂的纳米颗粒，并有望与细胞水平的条件和体内实验直接联系起来。纳米颗粒表面的蛋白质日冕导致受体结合基序的呈现，并定义了细胞之间的相互作用。在这里，作者使用抗体映射荧光量子点报告器和流式细胞术来衡量这些识别基序在生物介质中的可用性。
Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or ‘biomolecular corona' conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle–biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments. The protein corona on nanoparticle surfaces leads to presentation of receptor-binding motifs and defines the cellular interactions. Here, the authors use antibody mapping fluorescent quantum dot reporters, and flow cytometry to measure the availability of these recognition motifs in biological media.
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影响因子：
17.1
作者：
Albanese, Alexandre;Walkey, Carl D.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
DOI：
10.1039/ft9938904185
发表时间：
1993-12-07
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS
影响因子：
--
作者：
BEAUMONT, PC;JOHNSON, DG;PARSONS, BJ
通讯作者：
PARSONS, BJ
影响因子：
17.1
作者：
Casals, Eudald;Pfaller, Tobias;Puntes, Victor
通讯作者：
Puntes, Victor
影响因子：
3.1
作者：
Kreyling WG;Fertsch-Gapp S;Schäffler M;Johnston BD;Haberl N;Pfeiffer C;Diendorf J;Schleh C;Hirn S;Semmler-Behnke M;Epple M;Parak WJ
通讯作者：
Parak WJ
影响因子：
17.1
作者：
Hadjidemetriou, Marilena;Al-Ahmady, Zahraa;Kostarelos, Kostas
通讯作者：
Kostarelos, Kostas