Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds.
Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds.
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DOI：
10.1083/jcb.200103105
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发表时间：
2001-08-20
期刊：
影响因子：
--
通讯作者：
Bement WM
中科院分区：
文献类型：
--
作者：
Mandato CA;Bement WM
非洲爪蟾卵母细胞在质膜伤口周围聚集一系列的f -肌动蛋白和肌球蛋白2。我们使用共聚焦延时（四维）显微镜分析了活卵母细胞的这一过程。伤口的愈合需要一个典型的由f -肌动蛋白和肌球蛋白2组成的“收缩环”的组装和收缩。然而，这个环与5-10 μm宽的局部肌动蛋白和肌球蛋白2组装“区域”一致。该区在环之前形成，并可通过抑制皮质流动和收缩性与环分离。然而，区域的稳定和向前运动需要收缩性和收缩环，这可以从收缩性和流动中断后的区域动力学变化或实验诱导的收缩环断裂中看出。我们得出结论，伤口诱导的收缩阵列具有其特有的灵活性、速度和强度，这是由两种不同成分的组合输入：一个高动态区，其中肌凝蛋白2和肌动蛋白优先组装，以及一个稳定的收缩肌动蛋白环。
Xenopus oocytes assemble an array of F-actin and myosin 2 around plasma membrane wounds. We analyzed this process in living oocytes using confocal time-lapse (four-dimensional) microscopy. Closure of wounds requires assembly and contraction of a classic “contractile ring” composed of F-actin and myosin 2. However, this ring works in concert with a 5–10-μm wide “zone” of localized actin and myosin 2 assembly. The zone forms before the ring and can be uncoupled from the ring by inhibition of cortical flow and contractility. However, contractility and the contractile ring are required for the stability and forward movement of the zone, as revealed by changes in zone dynamics after disruption of contractility and flow, or experimentally induced breakage of the contractile ring. We conclude that wound-induced contractile arrays are provided with their characteristic flexibility, speed, and strength by the combined input of two distinct components: a highly dynamic zone in which myosin 2 and actin preferentially assemble, and a stable contractile actomyosin ring.
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DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA
N-乙基胺化的重霉素。肌动球蛋白相互作用的探针。
影响因子：
7.8
作者：
Meeusen, R L;Cande, W Z
通讯作者：
Cande, W Z
DOI：
10.1083/jcb.140.2.355
发表时间：
1998-01-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lippincott J;Li R
通讯作者：
Li R
DOI：
10.1083/jcb.131.5.1243
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gunsalus KC;Bonaccorsi S;Williams E;Verni F;Gatti M;Goldberg ML
通讯作者：
Goldberg ML
影响因子：
9.2
作者：
Jacinto, A;Wood, W;Martin, P
通讯作者：
Martin, P