Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila.
Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila.
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DOI：
10.1083/jcb.149.2.471
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发表时间：
2000-04-17
期刊：
影响因子：
--
通讯作者：
Montague RA
中科院分区：
文献类型：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
在形态发生和伤口愈合过程中，细胞形状变化和运动的分子和细胞基础引起了人们的强烈兴趣，而且才刚刚开始被理解。在这里，我们通过三种途径研究了在背侧闭合过程中负责形态发生的力量。首先，我们使用实时和延时激光共聚焦显微镜来跟踪肌动蛋白动力学，并记录活的、未受干扰的胚胎中的细胞形状变化和组织运动。我们用一个普遍表达的转基因标记细胞，该转基因编码GFP融合到苍蝇moesin的自主折叠肌动蛋白结合片段。其次，我们使用生物力学方法通过跟踪各种组织对紫外激光切割的响应来检查背侧闭合期间刚度/张力的分布。我们测试了之前的模型（Young, pe， A.M.）Richman， A.S. Ketchum和D.P. Kiehart, 1993。基因发育。7:29-41)，外侧表皮的前缘是一个可收缩的钱袋，为背部闭合提供力量。我们表明，这种结构在张力下表现为细胞上的钱包串，然而，我们提供的证据表明，它单独不能解释负责背闭合的力。此外，我们发现羊膜中存在各向同性的刚度/张力，而侧表皮中存在各向异性的刚度/张力。羊膜的张力可能有助于背侧闭合，但侧表皮的张力则相反。第三，我们通过反复消融羊膜层和外侧表皮前缘的细胞来研究各种组织在背侧闭合中的作用。我们的数据提供了强有力的证据，这两种组织似乎都有助于活胚胎正常的背闭合，但令人惊讶的是，这两种组织都不是绝对需要的背闭合。最后，我们证实了果蝇表皮在机械和紫外线激光损伤后都能快速、可再生地愈合，即使是那些反复照射的伤口。在愈合过程中，肌动蛋白被迅速招募到伤口边缘，在伤口愈合过程中，一个新形成的细胞上钱袋线收缩。这一结果确立了果蝇胚胎作为研究伤口愈合的良好系统。此外，我们的观察表明，这种昆虫表皮系统的伤口愈合与脊椎动物原位组织和培养的脊椎动物细胞的伤口愈合相似（参见Kiehart， D.P. 1999）。咕咕叫。医学杂志9:R602-R605)。
The molecular and cellular bases of cell shape change and movement during morphogenesis and wound healing are of intense interest and are only beginning to be understood. Here, we investigate the forces responsible for morphogenesis during dorsal closure with three approaches. First, we use real-time and time-lapsed laser confocal microscopy to follow actin dynamics and document cell shape changes and tissue movements in living, unperturbed embryos. We label cells with a ubiquitously expressed transgene that encodes GFP fused to an autonomously folding actin binding fragment from fly moesin. Second, we use a biomechanical approach to examine the distribution of stiffness/tension during dorsal closure by following the response of the various tissues to cutting by an ultraviolet laser. We tested our previous model (Young, P.E., A.M. Richman, A.S. Ketchum, and D.P. Kiehart. 1993. Genes Dev. 7:29–41) that the leading edge of the lateral epidermis is a contractile purse-string that provides force for dorsal closure. We show that this structure is under tension and behaves as a supracellular purse-string, however, we provide evidence that it alone cannot account for the forces responsible for dorsal closure. In addition, we show that there is isotropic stiffness/tension in the amnioserosa and anisotropic stiffness/tension in the lateral epidermis. Tension in the amnioserosa may contribute force for dorsal closure, but tension in the lateral epidermis opposes it. Third, we examine the role of various tissues in dorsal closure by repeated ablation of cells in the amnioserosa and the leading edge of the lateral epidermis. Our data provide strong evidence that both tissues appear to contribute to normal dorsal closure in living embryos, but surprisingly, neither is absolutely required for dorsal closure. Finally, we establish that the Drosophila epidermis rapidly and reproducibly heals from both mechanical and ultraviolet laser wounds, even those delivered repeatedly. During healing, actin is rapidly recruited to the margins of the wound and a newly formed, supracellular purse-string contracts during wound healing. This result establishes the Drosophila embryo as an excellent system for the investigation of wound healing. Moreover, our observations demonstrate that wound healing in this insect epidermal system parallel wound healing in vertebrate tissues in situ and vertebrate cells in culture (for review see Kiehart, D.P. 1999. Curr. Biol. 9:R602–R605).
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影响因子：
9.2
作者：
Liu, HZ;Su, YC;Skolnik, EY
通讯作者：
Skolnik, EY
影响因子：
64.8
作者：
MARTIN, P;LEWIS, J
通讯作者：
LEWIS, J
影响因子：
64.8
作者：
COOKE, J
通讯作者：
COOKE, J
影响因子：
7.8
作者：
Brock, J;Midwinter, K;Martin, P
通讯作者：
Martin, P
DOI：
10.1007/bf00848157
发表时间：
1984-01-01
期刊：
WILHELM ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
JURGENS, G;WIESCHAUS, E;KLUDING, H
通讯作者：
KLUDING, H