Photonic force optical coherence elastography for three-dimensional mechanical microscopy.
Photonic force optical coherence elastography for three-dimensional mechanical microscopy.
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DOI：
10.1038/s41467-018-04357-8
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发表时间：
2018-05-25
影响因子：
16.6
通讯作者：
Adie SG
中科院分区：
文献类型：
--
作者：
Leartprapun N;Iyer RR;Untracht GR;Mulligan JA;Adie SG
光学镊子是非接触式捕获和微操作的宝贵工具，但它们促进生物样品高通量体积微流变学用于机械生物学研究的能力受到与单个头部振荡激发和检测相关的精确对准的限制。相比之下，来自低数值孔径光束的辐射压力可以在扩展的深度范围内施加横向局部力。在这里，我们提出了光子力光学相干弹性成像（PF-OCE），利用相敏干涉检测来跟踪嵌入粘弹性水凝胶中的微珠在调制辐射压力诱导下的亚纳米振荡。由于由超低辐射压力力引起的位移通常被吸收介导的热效应所掩盖，因此在对水凝胶的光热响应进行独立测量和解耦后，可以分离出微珠的机械响应。在不同琼脂糖浓度的水凝胶中，用fp - oce对水凝胶的力学响应进行了体积成像，结果与剪切流变法对水凝胶的整体力学表征一致。光学镊子虽然非常适合于微观操作，但很难应用于体积微流变学。在这里，Leartprapun等人将低na光辐射压力力与敏感干涉检测相结合，使体积微流变学在生物系统中具有很好的应用前景。
Optical tweezers are an invaluable tool for non-contact trapping and micro-manipulation, but their ability to facilitate high-throughput volumetric microrheology of biological samples for mechanobiology research is limited by the precise alignment associated with the excitation and detection of individual bead oscillations. In contrast, radiation pressure from a low-numerical aperture optical beam can apply transversely localized force over an extended depth range. Here we present photonic force optical coherence elastography (PF-OCE), leveraging phase-sensitive interferometric detection to track sub-nanometer oscillations of beads, embedded in viscoelastic hydrogels, induced by modulated radiation pressure. Since the displacements caused by ultra-low radiation-pressure force are typically obscured by absorption-mediated thermal effects, mechanical responses of the beads were isolated after independent measurement and decoupling of the photothermal response of the hydrogels. Volumetric imaging of bead mechanical responses in hydrogels with different agarose concentrations by PF-OCE was consistent with bulk mechanical characterization of the hydrogels by shear rheometry. Optical tweezers, while well suited for micro-manipulation, are difficult to apply to volumetric microrheology. Here, Leartprapun et al. combine low-NA optical radiation-pressure forces with sensitive interferometric detection to enable volumetric microrheology with promising applications in biological systems.
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通讯作者：
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