In vitro synergy of isavuconazole in combination with colistin against Candida auris.
In vitro synergy of isavuconazole in combination with colistin against Candida auris.
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DOI：
10.1038/s41598-020-78588-5
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发表时间：
2020-12-08
影响因子：
4.6
通讯作者：
Dannaoui E
中科院分区：
文献类型：
--
作者：
Schwarz P;Bidaud AL;Dannaoui E
对15株临床分离的金黄色念珠菌，采用基于EUCAST抗真菌药敏试验方法的微量稀释法和琼脂扩散法，用含或不含粘菌素的RPMI琼脂扩散法检测了异武康唑与粘菌素的体外相互作用。用分数抑菌浓度指数和基于Bliss模型的响应面分析对棋盘结果进行解释。经棋盘检验，用分数抑菌浓度指数解释数据时，93%的菌株协同作用，响应面分析解释80%的菌株协同作用。通过琼脂扩散试验，虽然所有联合用药的最低抑菌浓度都低于单独使用异武康唑，但只有13%的分离物符合协同作用的定义。在 +/−2log2稀释度下，EUCAST法与梯度浓度条带法的基本符合率为93.3%。对于所使用的任何技术或解释模型，从未观察到拮抗相互作用。
The in vitro interactions of isavuconazole with colistin were evaluated against 15 clinical Candida auris isolates by a microdilution checkerboard technique based on the EUCAST reference method for antifungal susceptibility testing and by agar diffusion using isavuconazole gradient concentration strips with or without colistin incorporated RPMI agar. Interpretation of the checkerboard results was done by the fractional inhibitory concentration index and by response surface analysis based on the Bliss model. By checkerboard, combination was synergistic for 93% of the isolates when interpretation of the data was done by fractional inhibitory concentration index, and for 80% of the isolates by response surface analysis interpretation. By agar diffusion test, although all MICs in combination decreased compared to isavuconazole alone, only 13% of the isolates met the definition of synergy. Essential agreement of EUCAST and gradient concentration strip MICs at +/− 2 log2 dilutions was 93.3%. Antagonistic interactions were never observed for any technique or interpretation model used.
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影响因子：
4.9
作者：
Kontoyiannis, Dimitrios P.;Lewis, Russell E.;Singh, Nina
通讯作者：
Singh, Nina
DOI：
10.1007/s10096-013-1850-8
发表时间：
2013-08-01
影响因子：
4.5
作者：
MacCallum, D. M.;Desbois, A. P.;Coote, P. J.
通讯作者：
Coote, P. J.
影响因子：
4.9
作者：
Arendrup, M. C.;Prakash, Anupam;Chowdhary, Anuradha
通讯作者：
Chowdhary, Anuradha
影响因子：
4.1
作者：
Lim LM;Ly N;Anderson D;Yang JC;Macander L;Jarkowski A 3rd;Forrest A;Bulitta JB;Tsuji BT
通讯作者：
Tsuji BT
影响因子：
4.9
作者：
Dorsthorst, DTAT;Verweij, PE;Mouton, JW
通讯作者：
Mouton, JW