Long Non-Coding RNA NKILA Reduces Oral Squamous Cell Carcinoma Development Through the NF-KappaB Signaling Pathway.
Long Non-Coding RNA NKILA Reduces Oral Squamous Cell Carcinoma Development Through the NF-KappaB Signaling Pathway.
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DOI：
10.1177/1533033820960747
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发表时间：
2020-01
影响因子：
2.8
通讯作者：
Dai Q
中科院分区：
文献类型：
--
作者：
Hu D;Zhong T;Dai Q
新出现的研究发现，长的非编码RNA(LncRNAs)在癌症的发展中起着关键作用。本研究旨在探讨核因子-kappaB(NF-κB)与LncRNA相互作用在口腔鳞癌病理过程中的作用机制。采用RT-qPCR方法检测口腔鳞癌组织、癌旁组织、正常口腔黏膜角质形成细胞和口腔鳞癌细胞系中NKILA的表达。选择KB细胞进行后续实验。通过口腔鳞癌细胞中NKILA功能的获得和丧失，确定NKILA在细胞增殖、迁移、侵袭和NF-κB信号通路中的作用。此外，通过诱导裸鼠移植瘤来确定NKILA在体外的作用。NKILA在口腔鳞癌组织和细胞中低表达。在高表达NKILA的细胞中，细胞的增殖、侵袭和迁移、肿瘤体积和重量受到显著抑制，而沉默NKILA则导致相反的趋势。此外，在高表达NKILA的细胞中，p-IκBα和核P65的蛋白水平显著降低，而IκBα和细胞质P65的蛋白水平显著升高。本研究为NKILA通过抑制NF-κB信号通路减少口腔鳞癌细胞的增殖、侵袭和迁移提供了证据。这一发现可能为口腔鳞癌的预防和治疗提供新的见解。
Emerging studies have identified that long non-coding RNAs (lncRNAs) play critical roles in cancer development. This study aims to explore the mechanism of NF-KappaB (NF-κB) interacting lncRNA (NKILA) in the pathological process of oral squamous cell carcinoma (OSCC). NKILA expression in OSCC tissues, paracancerous tissues, and normal human oral keratinocytes and OSCC cell lines was detected using RT-qPCR. KB cells were selected for the follow-up experiments. The role of NKILA in cell proliferation, migration, invasion, and NF-κB signaling pathway was identified using the gain- and loss-of function of NKILA in OSCC cells. Additionally, the role of NKILA in vitro was determined by inducing xenograft tumors in nude mice. NKILA was poorly expressed in OSCC tissues and cells. Cell proliferation, invasion and migration, tumor volume and weight were significantly suppressed in cells with overexpressed NKILA, while silencing NKILA led to opposite trends. Moreover, the protein levels of p-IκBα and nuclear-p65 were markedly decreased, while the levels of IκBα and cytoplasm-p65 were enhanced in cells with overexpressed NKILA. This study provided evidence that NKILA could reduce proliferation, invasion and migration of OSCC cells through inhibiting the NF-κB signaling pathway. The findings may offer new insights for OSCC prevention and treatment.
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DOI：
10.1007/s00109-018-1621-1
发表时间：
2018-04
期刊：
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