Detection of alveolar fibrocytes in idiopathic pulmonary fibrosis and systemic sclerosis.
Detection of alveolar fibrocytes in idiopathic pulmonary fibrosis and systemic sclerosis.
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DOI：
10.1371/journal.pone.0053736
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Crestani B
中科院分区：
文献类型：
--
作者：
Borie R;Quesnel C;Phin S;Debray MP;Marchal-Somme J;Tiev K;Bonay M;Fabre A;Soler P;Dehoux M;Crestani B
纤维细胞是成纤维细胞的循环前体。特发性肺纤维化（IPF）患者的血纤维细胞增加。本研究的目的是确定是否肺泡纤维细胞检测支气管肺泡灌洗（BAL），以确定其预后价值，以及其潜在的关联与培养的成纤维细胞从BAL。我们定量了26例IPF患者、9例系统性硬化症（SSc）-间质性肺病（ILD）患者和11例对照者的BAL中的纤维细胞。培养BAL细胞以分离肺泡成纤维细胞。在14/26例IPF（54%）和5/9例SSc患者（55%）的BAL中检测到纤维细胞，而在对照组中从未检测到纤维细胞。在IPF和SSc-ILD患者中，纤维细胞分别占BAL细胞的中位数2.5% [0.4-19.7]和3.0% [2.7-3.7]。在特发性肺纤维化患者中，肺泡纤维细胞的数量与肺泡巨噬细胞的数量相关，并且与较轻的疾病相关，但与更好的结局无关。在12/26例IPF（46%）、5/9例SSc-ILD（65%）中从BAL培养成纤维细胞，而在对照组中从未培养成纤维细胞。BAL纤维细胞的检测并不能预测成纤维细胞的阳性培养。在大约一半的IPF和SSc-ILD患者的BAL液中检测到纤维细胞。它们的数量与IPF患者中不太严重的疾病相关，并且与从BAL液中生长成纤维细胞的能力无关。
Fibrocytes are circulating precursors for fibroblasts. Blood fibrocytes are increased in patients with idiopathic pulmonary fibrosis (IPF). The aim of this study was to determine whether alveolar fibrocytes are detected in broncho-alveolar lavage (BAL), to identify their prognostic value, and their potential association with culture of fibroblasts from BAL. We quantified fibrocytes in BAL from 26 patients with IPF, 9 patients with Systemic Sclerosis(SSc)-interstitial lung disease (ILD), and 11 controls. BAL cells were cultured to isolate alveolar fibroblasts. Fibrocytes were detected in BAL in 14/26 IPF (54%) and 5/9 SSc patients (55%), and never in controls. Fibrocytes were in median 2.5% [0.4–19.7] and 3.0% [2.7–3.7] of BAL cells in IPF and SSc-ILD patients respectively. In IPF patients, the number of alveolar fibrocytes was correlated with the number of alveolar macrophages and was associated with a less severe disease but not with a better outcome. Fibroblasts were cultured from BAL in 12/26 IPF (46%), 5/9 SSc-ILD (65%) and never in controls. The detection of BAL fibrocytes did not predict a positive culture of fibroblasts. Fibrocytes were detected in BAL fluid in about half of the patients with IPF and SSc-ILD. Their number was associated with less severe disease in IPF patients and did not associate with the capacity to grow fibroblasts from BAL fluid.
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DOI：
10.1186/1755-1536-4-15
发表时间：
2011-07-01
期刊：
Fibrogenesis & tissue repair
影响因子：
--
作者：
Tourkina E;Bonner M;Oates J;Hofbauer A;Richard M;Znoyko S;Visconti RP;Zhang J;Hatfield CM;Silver RM;Hoffman S
通讯作者：
Hoffman S
DOI：
10.1038/nri2990
发表时间：
2011-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.3
作者：
Medina, Abelardo;Ghahary, Aziz
通讯作者：
Ghahary, Aziz
DOI：
10.1152/ajplung.00096.2007
发表时间：
2008-02-01
影响因子：
4.9
作者：
Quesnel, Christophe;Marchand-Adam, Sylvain;Dehoux, Monique
通讯作者：
Dehoux, Monique
DOI：
10.1513/pats.200512-133tk
发表时间：
2006-06-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Lama, Vibha N;Phan, Sem H
通讯作者：
Phan, Sem H