Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro.
Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro.
复制标题
在体外，后肾间充质细胞的增殖受到 miR-743a 介导的 WT1 抑制的抑制。
DOI：
10.3892/mmr.2016.5762
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发表时间：
2016-11
影响因子：
3.4
通讯作者：
QIN ZHOU
中科院分区：
文献类型：
--
作者：
MINGMING XUE;YURU ZHOU;XIAOYU LIU;DONGSHENG NI;YANXIA HU;YAOSHUI LONG;PAN JU;QIN ZHOU
为了寻找潜在的靶向Wilms肿瘤抑制因子1 （WT1）的miRNAs (miRNAs)， WT1是祖细胞增殖和肾脏正常发育所需的转录因子，并阐明miRNAs对WT1的影响，我们首先分析了WT1的3'-非翻译区（3' - UTR），并鉴定了miR - 743a，这是一种很少报道的miRNA。在本文中，荧光素酶报告基因检测证实miR - 743a能够直接靶向WT1的3' - UTR。随后进行逆转录-定量聚合酶链反应，结合western blotting分析，结果显示WT1在mRNA和蛋白水平上均受到显著抑制。此外，一项5 -乙基- 2' -脱氧尿苷（EdU）细胞增殖试验，结合WT1拯救策略，表明miR - 743a抑制后肾间充质（MM）细胞的增殖，部分原因是通过靶向WT1。总之，miR - 743a通过靶向WT1，在体外抑制MM细胞的增殖，可能在肾脏发育和肾脏相关疾病中具有重要功能。
To seek out the potential microRNAs (miRNAs) that target Wilms' tumor suppressor 1 (WT1), a transcription factor required for progenitor proliferation as well as normal development of the kidney, and to clarify the effects of the miRNAs on WT1, the 3'-untranslated region (3'‑UTR) of WT1 was initially analyzed and miR‑743a, a seldom‑reported miRNA, was identified. In the present paper, luciferase reporter assays were performed to confirm that miR‑743a is able to directly target the 3'‑UTR of WT1. Subsequently, reverse transcription‑quantitative polymerase chain reaction, combined with western blotting analyses, were performed, and the results revealed a significant inhibition of WT1 at the mRNA and the protein levels. Furthermore, a 5‑ethynyl‑2'‑deoxyuridine (EdU) cell proliferation assay, coupled with a WT1 rescue strategy, demonstrated that miR‑743a inhibited the proliferation of metanephric mesenchymal (MM) cells, in part by targeting WT1. In conclusion, by targeting WT1, miR‑743a suppresses the proliferation of MM cells in vitro, and probably possesses vital functions in kidney development and kidney‑associated diseases.
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影响因子：
3.6
作者：
Lv, Xiaoyan;Mao, Zhaomin;Zhou, Qin
通讯作者：
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影响因子：
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作者：
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DOI：
10.1152/ajprenal.00076.2015
发表时间：
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影响因子：
13.6
作者：
Ho, Jacqueline;Kreidberg, Jordan A.
通讯作者：
Kreidberg, Jordan A.
影响因子：
4.3
作者：
Berry RL;Ozdemir DD;Aronow B;Lindström NO;Dudnakova T;Thornburn A;Perry P;Baldock R;Armit C;Joshi A;Jeanpierre C;Shan J;Vainio S;Baily J;Brownstein D;Davies J;Hastie ND;Hohenstein P
通讯作者：
Hohenstein P