The Effects of Sevoflurane on Population Spikes in CA1 and Dentate Gyrus of the Rat Hippocampus In Vitro
The Effects of Sevoflurane on Population Spikes in CA1 and Dentate Gyrus of the Rat Hippocampus In Vitro
复制标题
七氟醚对离体大鼠海马CA1区和齿状回群体峰值的影响
作者：
K. Hirota;S. Roth
本实验研究了七氟醚对大鼠海马两条突触通路群体峰（PSs）的影响。刺激电极分别放置在Schaffer侧支纤维或穿孔通路上激活CA1锥体神经元或齿状回（DG）神经元的输入。细胞外玻璃微电极记录PSs。采用配对脉冲刺激诱导配对脉冲促进（PPF）。七氟醚（0.4-5.0 vol%）显著降低CA1和DG的PSs幅度，且呈剂量依赖性和可逆性（25%有效剂量值分别为4.1和0.9 vol%）。PS振幅的刺激-反应关系表明，七氟醚增加了CA1和DG中PS产生的阈值。七氟醚（2.0 vol%）使CA1和DG的PPF分别从127%和263%提高到153%和494%。结果表明，七氟醚对DG中PSs的影响大于CA1神经元，七氟醚增强了CA1和DG中的PPF，并且七氟醚的作用与以前报道的其他挥发性或静脉麻醉药在海马制剂中的作用不同。意义：挥发性麻醉剂七氟醚以不同于其他全身麻醉剂的方式改变海马中单个通路的神经兴奋性。结果与全身麻醉的部位特异性作用机制一致。（Anesth Analg 1997;85:426-30）
We studied the effects of sevoflurane on population spikes (PSs) in two synaptic pathways in rat hippocampal slices.Stimulating electrodes were placed on Schaffer collateral fibers or perforant path to activate inputs to CA1 pyramidal neurons or dentate gyrus (DG) neurons, respectively. Extracellular glass microelectrodes were used to record PSs. The paired-pulse stimulus was used to induce the paired-pulse facilitation (PPF). Sevoflurane (0.4-5.0 vol%) significantly decreased the amplitudes of PSs of CA1 and DG in a dose-dependent and reversible manner (25% effective dose values were 4.1 and 0.9 vol%, respectively). The stimulus-response relationships for PS amplitudes revealed that sevoflurane increased the threshold for PS generation in CA1 and DG. Sevoflurane (2.0 vol%) significantly enhanced PPF from 127% and 263% to 153% and 494% in CA1 and DG, respectively. The results imply that the effects of sevoflurane on PSs are greater in DG than in CA1 neurons, that sevoflurane enhances the PPF in both CA1 and DG, and that the actions of sevoflurane are not similar to those of other volatile or intravenous anesthetics previously reported in hippocampal preparations. Implications: The volatile anesthetic sevoflurane alters neural excitability of individual pathways in the hippocampus in a manner different from other general anesthetics. The results are consistent with a site-specific mechanism of action for general anesthesia. (Anesth Analg 1997;85:426-30)
挥发性麻醉剂对大鼠海马突触传递的影响。
影响因子：
8.8
作者：
Pearce,RA;Stringer,JL;Lothman,EW
通讯作者：
Lothman,EW