Characteristics of the Genome, Transcriptome and Ganoderic Acid of the Medicinal Fungus Ganoderma lingzhi.
Characteristics of the Genome, Transcriptome and Ganoderic Acid of the Medicinal Fungus Ganoderma lingzhi.
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药用真菌灵芝基因组、转录组及灵芝酸特征
DOI：
10.3390/jof8121257
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发表时间：
2022-11-28
期刊：
影响因子：
4.7
通讯作者：
Liu, Gaoqiang
中科院分区：
文献类型：
--
作者：
Wu, Qiang;Liu, Huan;Shi, Yixin;Li, Wanting;Huang, Jia;Xue, Feifei;Liu, Yongnan;Liu, Gaoqiang
灵芝（灵芝科）是一种可食用和药用蘑菇属，产生多种生物活性化合物。灵芝因其药用价值在中国已有2000多年的历史。然而，凌芝的基因组信息尚未被表征。在这里，我们对其49.15 mb的基因组进行了表征，编码了13,125个预测基因，并通过Illumina和PacBio平台进行了测序。在灵芝中鉴定出了广谱的碳水化合物活性酶，共鉴定出519种。然后，对参与性别识别和灵芝酸（GA，关键生物活性代谢物）生物合成的基因进行了表征。此外，我们利用UPLC-ESI-MS/MS鉴定并推断了20个GA主要成分的可能结构，包括一个新的特殊甘露酸a。此外，从RNA-Seq数据中发现了3996个新的转录本，并预测了9276个基因具有选择性剪接的可能性。这些备选剪接基因被KEGG富集为涉及蛋白质加工、内吞作用和代谢活动的功能类别。这些基因组学、转录组学和GA成分资源将丰富灵芝生物学、遗传和次生代谢途径研究的工具箱。
Ganoderma (Ganodermaceae) is a genus of edible and medicinal mushrooms that create a diverse set of bioactive compounds. Ganoderma lingzhi has been famous in China for more than 2000 years for its medicinal properties. However, the genome information of G. lingzhi has not been characterized. Here, we characterized its 49.15-Mb genome, encoding 13,125 predicted genes which were sequenced by the Illumina and PacBio platform. A wide spectrum of carbohydrate-active enzymes, with a total number of 519 CAZymes were identified in G. lingzhi. Then, the genes involved in sexual recognition and ganoderic acid (GA, key bioactive metabolite) biosynthesis were characterized. In addition, we identified and deduced the possible structures of 20 main GA constituents by UPLC-ESI-MS/MS, including a new special ganochlearic acid A. Furthermore, 3996 novel transcripts were discovered, and 9276 genes were predicted to have the possibility of alternative splicing from RNA-Seq data. The alternative splicing genes were enriched for functional categories involved in protein processing, endocytosis, and metabolic activities by KEGG. These genomic, transcriptomic, and GA constituents’ resources would enrich the toolbox for biological, genetic, and secondary metabolic pathways studies in G. lingzhi.
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影响因子：
3.7
作者：
Huang YH;Wu HY;Wu KM;Liu TT;Liou RF;Tsai SF;Shiao MS;Ho LT;Tzean SS;Yang UC
通讯作者：
Yang UC
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
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影响因子：
2.8
作者：
Liu, Yong-Nan;Tong, Tian;Liu, Gao-Qiang
通讯作者：
Liu, Gao-Qiang
影响因子：
3.9
作者：
Peng, Xing-Rong;Wang, Xia;Qiu, Ming-Hua
通讯作者：
Qiu, Ming-Hua
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.