Image reconstruction in non-reciprocal broken-ray tomography.
Image reconstruction in non-reciprocal broken-ray tomography.
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DOI：
10.1364/josaa.461150
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发表时间：
2022-09-01
期刊：
影响因子：
--
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文献类型：
--
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光学方法的生物医学断层成像是相当大的兴趣，由于其非侵入性的性质和敏感性的生理上重要的标志物。与其他成像方式类似，光学方法可以通过利用外源性造影剂来增强。通常，这些是荧光分子，其可以由于各种机制而聚集在感兴趣的区域中。在当前的成像方法中，分别确定内在（与组织相关）和外在（与造影剂相关）光学参数。这可能导致错误，特别是由于使用简化的启发式模型的光谱依赖性的光学参数。最近，我们发展了非互易破碎射线层析成像（NRBRT）的弱散射系统的荧光成像的理论。NRBRT能够同时重建的荧光团浓度，以及在激发和发射波长的固有光衰减系数。重要的是，在NRBRT中没有关于组织光学特性的光谱依赖性的假设。在这项研究中，我们进行数值验证的NRBRT在现实条件下使用蒙特卡罗方法生成的正演数据。我们证明，NRBRT可用于高达四个散射长度的大小的样品的断层成像。研究了探测器的面积、接收角等物理特性对探测器性能的影响。
Optical methods of biomedical tomographic imaging are of considerable interest due to their non-invasive nature and sensitivity to physiologically important markers. Similarly to other imaging modalities, optical methods can be enhanced by utilizing extrinsic contrast agents. Typically, these are fluorescent molecules, which can aggregate in regions of interest due to various mechanisms. In the current approaches to imaging, the intrinsic (related to the tissue) and extrinsic (related to the contrast agent) optical parameters are determined separately. This can result in errors, in particular, due to using simplified heuristic models for the spectral dependence of the optical parameters. Recently, we have developed the theory of non-reciprocal broken-ray tomography (NRBRT) for fluorescence imaging of weakly scattering systems. NRBRT enables simultaneous reconstruction of the fluorophore concentration as well as of the intrinsic optical attenuation coefficient at both the excitation and the emission wavelengths. Importantly, no assumption about the spectral dependence of the tissue optical properties is made in NRBRT. In this study, we perform numerical validation of NRBRT under realistic conditions using the Monte Carlo method to generate forward data. We demonstrate that NRBRT can be used for tomographic imaging of samples of up to four scattering lengths in size. The effects of physical characteristics of the detectors such as the area and the acceptance angle are also investigated.
同时进行多波长层状光学断层扫描。
影响因子：
3.6
作者：
Burgess SA;Bouchard MB;Yuan B;Hillman EM
通讯作者：
Hillman EM