Integration of multiparameter flow cytometry score improves prognostic stratification provided by standard models in primary myelofibrosis.
Integration of multiparameter flow cytometry score improves prognostic stratification provided by standard models in primary myelofibrosis.
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多参数流式细胞术评分的整合改善了原发性骨髓纤维化标准模型提供的预后分层。
DOI：
10.1002/ajh.26548
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发表时间：
2022-07
影响因子：
12.8
通讯作者：
Vannucchi, Alessandro M.
中科院分区：
文献类型：
--
作者：
Mannelli, Francesco;Bencini, Sara;Coltro, Giacomo;Loscocco, Giuseppe G.;Peruzzi, Benedetta;Rotunno, Giada;Maccari, Chiara;Gesullo, Francesca;Borella, Miriam;Paoli, Chiara;Caporale, Roberto;Mannarelli, Carmela;Annunziato, Francesco;Guglielmelli, Paola;Vannucchi, Alessandro M.
骨髓纤维化（MF）的预后建模传统上追求信息性临床和血液学参数的整合，以分离具有不同结局的患者类别。现代分层还包括来自核型和突变的遗传数据。然而，一些标准化较差的变量，如外周血（PB）原始细胞计数的形态，仍然包括在内。在这项研究中，我们使用多参数流式细胞术（MFC），目的是提高现有分数的性能。我们研究了363例MF患者，在诊断时使用MFC文件进行PB CD 34+细胞计数测定。我们根据MF背景调整Ogata评分，包括2个参数：绝对CD 34+细胞计数（/μL）和粒细胞与淋巴细胞SSC比。1分归因于每个参数的阈值以上。因此，患者被分类为MFClow（评分= 0，62.0%）、MFCint（评分= 1，29.5%）和MFChigh（评分= 2，8.5%）。与MFCint（55个月）和MFChigh（19个月）相比，MFClow的中位OS（未达到）显著更长。我们将MFC整合到已建立的模型中，作为形态学PB原始细胞计数的替代。根据MFC增强评分对患者进行重新分类，并比较一致性（C）指数。关于IPSS，标准和MFC增强模型的C指数分别为0.67和0.74（Z评分-3.82; p = 0.0001）。PMF患者的MFC增强MIPSS 70+模型产生的C指数为0.78，优于其标准对应物（C指数0.73; Z评分-2.88，p = 0.004）。我们的数据表明，MFC衍生的参数，可以很容易地从用于CD 34+细胞测定的标准测定中获得，可能有助于完善目前骨髓纤维化的预后分层模型。
Prognostic modeling in myelofibrosis (MF) has classically pursued the integration of informative clinical and hematological parameters to separate patients' categories with different outcomes. Modern stratification includes also genetic data from karyotype and mutations. However, some poorly standardized variables, as peripheral blood (PB) blast count by morphology, are still included. In this study, we used multiparameter flow cytometry (MFC) with the aim of improving performance of existing scores. We studied 363 MF patients with available MFC files for PB CD34+ cells count determination at diagnosis. We adapted Ogata score to MF context including 2 parameters: absolute CD34+ cells count (/μL) and granulocytes to lymphocytes SSC ratio. A score of 1 was attributed to above‐threshold values of each parameter. Accordingly, patients were categorized as MFClow (score = 0, 62.0%), MFCint (score = 1, 29.5%), and MFChigh (score = 2, 8.5%). MFClow had significantly longer median OS (not reached) compared to MFCint (55 months) and MFChigh (19 months). We integrated MFC into established models as a substitute of morphological PB blasts count. Patients were reclassified according to MFC‐enhanced scores, and concordance (C‐) indexes were compared. As regards IPSS, C‐indexes were 0.67 and 0.74 for standard and MFC‐enhanced model, respectively (Z score − 3.82; p = 0.0001). MFC‐enhanced MIPSS70+ model in PMF patients yielded a C‐index of 0.78, outperforming its standard counterpart (C‐index 0.73; Z score − 2.88, p = 0.004). Our data suggest that the incorporation of MFC‐derived parameters, easily attainable from standard assay used for CD34+ cells determination, might help to refine the current prognostic stratification models in myelofibrosis.
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影响因子：
20.3
作者：
Vardiman, James W.;Thiele, Juergen;Bloomfield, Clara D.
通讯作者：
Bloomfield, Clara D.
影响因子：
5.2
作者：
Mannelli F;Bencini S;Piccini M;Gianfaldoni G;Bonetti MI;Peruzzi B;Caporale R;Scappini B;Pancani F;Ponziani V;Signori L;Zizza M;Annunziato F;Bosi A
通讯作者：
Bosi A
DOI：
10.1089/scd.1.1996.5.213
发表时间：
1996-06-01
期刊：
Journal of hematotherapy
影响因子：
--
作者：
Sutherland, D R;Anderson, L;Chin-Yee, I
通讯作者：
Chin-Yee, I
影响因子：
3.4
作者：
Matarraz, Sergio;Lopez, Antonio;Orfao, Alberto
通讯作者：
Orfao, Alberto
影响因子：
12.8
作者：
Tefferi, Ayalew;Partain, Daniel K.;Patnaik, Mrinal M.
通讯作者：
Patnaik, Mrinal M.