Fluorite Dissolution by a Phosphate-Solubilizing Bacterium Isolated from Groundwater of Xianghuangqi County, Inner Mongolia, China
Fluorite Dissolution by a Phosphate-Solubilizing Bacterium Isolated from Groundwater of Xianghuangqi County, Inner Mongolia, China
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内蒙古镶黄旗县地下水中分离的解磷细菌对萤石的溶解
DOI：
10.1080/01490451.2019.1654042
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发表时间：
2019-08
影响因子：
2.3
通讯作者：
Tatsuo Iwasa
中科院分区：
文献类型：
--
作者：
Gang Dai;Wen Durige;Xiong Geng;Chao Luomeng;Tatsuo Iwasa
内蒙古镶黄旗是我国典型的高氟地区。本文探讨了溶磷菌在该地区高氟地下水形成中的可能作用。从当地地下水中分离出一种土著光合细菌。16 S rDNA序列分析表明该菌属于假单胞菌属。这种细菌在磷酸盐限制条件下引起培养基酸化。HPLC分析检测到几种可能酸化培养基的代谢物。该菌在含有2 mg/L、20 mg/L和200 mg/L葡萄糖的基本培养基中进行的氟石溶解实验表明，葡萄糖的初始浓度对培养基的pH值和氟石溶解率有很大影响。细菌无细胞培养基的pH在整个实验中保持恒定。PSB的引入引起pH的初始降低，随后增加。对于用200 mg/L、20 mg/L和2 mg/L葡萄糖修正的培养基，测得的最小pH值分别为4.8、5.8和6.0。观察到细菌无细胞培养基中的氟浓度缓慢增加，并且接种PSB增加了氟释放的速率，导致细菌接种培养基中的氟浓度明显超过对照培养基中的氟浓度。在添加200 mg/L葡萄糖的培养基中观察到最高的萤石溶解速率，而在添加20 mg/L和2 mg/L葡萄糖的培养基中观察到的速率相当。鉴于光合细菌在自然环境中的普遍存在，这些发现表明光合细菌可能是高氟地区氟动员的重要贡献者。
Abstract Xianghuangqi County of Inner Mongolia is a typical high-fluoride area in China. Present work discussed a possible role of phosphate-solubilizing bacteria (PSBs) in the formation of high-fluoride groundwater in this area. An indigenous PSB was isolated from the local groundwater. Analysis of 16S rDNA sequence indicates that the bacterium belongs to the Pseudomonas genus. This bacterium caused acidification of the culture media under phosphate-limited conditions. HPLC analysis detected several metabolites that are likely to acidify the cultural media. Fluorite dissolution experiment with this bacterium in minimum medium containing 2 mg/L, 20 mg/L, and 200 mg/L glucose showed that the initial concentration of glucose has a large effect on pH of the culture medium and the fluorite dissolution rate. The pH of bacterial cell-free medium remained constant throughout the experiment. Introduction of the PSB caused an initial decrease in pH, followed by an increase. The measured pH minima were 4.8, 5.8, and 6.0 for culture media amended with 200 mg/L, 20 mg/L, and 2 mg/L glucose, respectively. A slow increase of the fluoride concentration in bacterial cell-free medium was observed, and inoculation of the PSB increased the rate of fluoride release causing the concentration of fluoride in bacterial-inoculated medium to markedly exceed that found in control medium. The highest fluorite dissolution rate was observed in culture medium amended with 200 mg/L glucose, while the rates observed in culture medium amended with 20 mg/L and 2 mg/L glucose were comparable. Given the ubiquity of PSBs in natural environment, these findings show PSBs could be important contributors to fluoride mobilization in high fluoride area.
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影响因子：
2.8
作者：
L. Latrach Tlemçani;D. Corroler;D. Barillier;R. Mosrati
通讯作者：
L. Latrach Tlemçani;D. Corroler;D. Barillier;R. Mosrati
DOI：
10.2307/3898388
发表时间：
1982-03
期刊：
Journal of Range Management
影响因子：
--
作者：
J. Kubota;E. A. Naphan;G. H. Oberly
通讯作者：
J. Kubota;E. A. Naphan;G. H. Oberly
影响因子：
5
作者：
C. Amrhein;D. Suarez
通讯作者：
C. Amrhein;D. Suarez
影响因子：
0.8
作者：
Zilong Li;Y. Tainosho;K. Shiraishi;M. Owada
通讯作者：
Zilong Li;Y. Tainosho;K. Shiraishi;M. Owada
影响因子：
5
作者：
D. Nordstrom;J. W. Ball;R. Donahoe;D. Whittemore
通讯作者：
D. Nordstrom;J. W. Ball;R. Donahoe;D. Whittemore