Topology Studies of the Chloroplast Protein Import Channel Toc75
Topology Studies of the Chloroplast Protein Import Channel Toc75
复制标题
叶绿体蛋白输入通道 Toc75 的拓扑研究
DOI：
10.1515/bc.2000.089
复制
发表时间：
2000
期刊：
影响因子：
27.5
通讯作者：
Enrico Schleiff
中科院分区：
文献类型：
--
作者：
N. Sveshnikova;Rudolf Grimm;Jürgen Soll;Enrico Schleiff
摘要研究蛋白质跨膜转运的一个主要目标是研究转运子亚基的结构和调控。我们分析了Toc 75，一个孔形成亚基的叶绿体外被膜的易位子。将Toc 75过表达并重构为脂质体。脂质体重构的Toc 75的免疫沉淀指示Toc 75的Nin-Cin取向。使用特定蛋白酶结合氨基酸测序对存在于外囊泡中的Toc 75进行有限的蛋白水解消化来研究Toc 75的拓扑结构。最后，基于已知蛋白质结构的计算机建模表明，Toc 75穿过具有16个两亲性β折叠的外包膜，并给出了拓扑模型。
Abstract A major goal in understanding protein transport across membranes is the investigation of the structure and regulation of the translocon subunits. We analysed Toc75, a pore-forming subunit of the translocon of the outer envelope of chloroplasts. Toc75 was over-expressed and reconstituted into liposomes. Immunoprecipitation of liposome-reconstituted Toc75 indicates an Nin-Cin orientation of Toc75. Limited proteolytic digestion of Toc75 present in outer envelope vesicles with specific proteases combined with amino acid sequencing was used to study the topology of Toc75. Finally, computer modelling based on known protein structures indicates that Toc75 traverses the outer envelope with 16 amphiphilic beta sheets and the topology model is presented.
DOI：
10.1016/0005-2736(91)90295-j
发表时间：
1991-01-30
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MACDONALD, RC;MACDONALD, RI;HU, LR
通讯作者：
HU, LR