Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes.
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes.
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DOI：
10.3791/54466
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发表时间：
2016-10-15
期刊：
影响因子：
--
通讯作者：
Lou X
中科院分区：
文献类型：
--
作者：
Ji C;Lou X
Phosphoinositides in the cell membrane are signaling lipids with multiple cellular functions. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a determinant phosphoinositide of the plasma membrane (PM), and it is required to modulate ion channels, actin dynamics, exocytosis, endocytosis, intracellular signaling, and many other cellular processes. However, the spatial organization of PI(4,5)P2 in the PM is controversial, and its nanoscale distribution is poorly understood due to the technical limitations of research approaches. Here by utilizing single molecule localization microscopy and the Pleckstrin Homology (PH) domain based dual color fluorescent probes, we describe a novel method to visualize the nanoscale distribution of PI(4,5)P2 in the PM in fixed membrane sheets as well as live cells.
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DOI：
10.1042/bj20090428
发表时间：
2009-07-29
期刊：
The Biochemical journal
影响因子：
--
作者：
Hammond GR;Schiavo G;Irvine RF
通讯作者：
Irvine RF
影响因子：
64.8
作者：
Dyachok, O;Isakov, Y;Tengholm, A
通讯作者：
Tengholm, A
DOI：
10.1073/pnas.1318188110
发表时间：
2013-11-12
影响因子：
11.1
作者：
Nan, Xiaolin;Collisson, Eric A.;Chu, Steven
通讯作者：
Chu, Steven
影响因子：
5.3
作者：
Milosevic, I;Sorensen, JB;Neher, E
通讯作者：
Neher, E
影响因子：
5.3
作者：
Huang, SH;Lifshitz, L;Czech, MP
通讯作者：
Czech, MP