patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120
patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120
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patD是一个受NtcA调控的基因，参与蓝藻鱼腥藻异囊频率的优化。
DOI：
10.1128/jb.00457-19
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发表时间：
2019-08
影响因子：
3.2
通讯作者：
Zhang Cheng Cai
中科院分区：
文献类型：
--
作者：
Wang Li;Lin Gui Ming;Niu Tian Cai;Zhang Shao Ran;Zhang Ju Yuan;Tang Guo Fang;Chen Wenli;Zhang Cheng Cai
Microorganisms have evolved various strategies in order to adapt to the environment and compete with other organisms. Heterocyst differentiation is a prokaryotic model for studying complex cellular regulation. The NtcA-regulated gene patD controls the ratio of heterocysts relative to vegetative cells on the filaments of Anabaena sp. strain PCC 7120. Such a regulation provides a mechanism through which carbon fixation by vegetative cells and nitrogen fixation by heterocysts are properly balanced to ensure optimal growth and keep a competitive edge for long-term survival. ABSTRACT In the filamentous multicellular cyanobacterium Anabaena sp. strain PCC 7120, 5 to 10% of the cells differentiate into heterocysts, which are specialized in N2 fixation. Heterocysts and vegetative cells are mutually dependent for filament growth through nutrient exchange. Thus, the heterocyst frequency should be optimized to maintain the cellular carbon and nitrogen (C/N) balance for filament fitness in the environment. Here, we report the identification of patD, whose expression is directly activated in developing cells by the transcription factor NtcA. The inactivation of patD increases heterocyst frequency and promotes the upregulation of the positive regulator of heterocyst development hetR, whereas its overexpression decreases the heterocyst frequency. The change in heterocyst frequency resulting from the inactivation of patD leads to the reduction in competitiveness of the filaments under combined-nitrogen-depleted conditions. These results indicate that patD regulates heterocyst frequency in Anabaena sp. PCC 7120, ensuring its optimal filament growth. IMPORTANCE Microorganisms have evolved various strategies in order to adapt to the environment and compete with other organisms. Heterocyst differentiation is a prokaryotic model for studying complex cellular regulation. The NtcA-regulated gene patD controls the ratio of heterocysts relative to vegetative cells on the filaments of Anabaena sp. strain PCC 7120. Such a regulation provides a mechanism through which carbon fixation by vegetative cells and nitrogen fixation by heterocysts are properly balanced to ensure optimal growth and keep a competitive edge for long-term survival.
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影响因子：
6.4
作者：
Nürnberg DJ;Mariscal V;Bornikoel J;Nieves-Morión M;Krauß N;Herrero A;Maldener I;Flores E;Mullineaux CW
通讯作者：
Mullineaux CW
影响因子：
2.6
作者：
Yang Yang-Yang;Xiao-Zhen Huang;Li Wang;V. Risoul;Cheng‐Cai Zhang;Wen-Li Chen
通讯作者：
Yang Yang-Yang;Xiao-Zhen Huang;Li Wang;V. Risoul;Cheng‐Cai Zhang;Wen-Li Chen
影响因子：
3.6
作者：
T. Black;Yuping Cai;C. Wolk
通讯作者：
T. Black;Yuping Cai;C. Wolk
氮饥饿信号 2-酮戊二酸对全局转录因子 NtcA 进行变构控制的结构基础
DOI：
10.1073/pnas.1001556107
发表时间：
2010-06
影响因子：
11.1
作者：
Teng, Yan-Bin;Zhao, Meng-Xi;Chen, Yi-Fei;He, Yong-Xing;Chen, Yuxing;Zhang, Cheng-Cai;Jiang, Yong-Liang;Zhou, Cong-Zhao
通讯作者：
Zhou, Cong-Zhao
影响因子：
3.6
作者：
Higa, Kelly C.;Rajagopalan, Ramya;Callahan, Sean M.
通讯作者：
Callahan, Sean M.