Microarrays reveal early transcriptional events during the termination of larval diapause in natural populations of the mosquito, Wyeomyia smithii.
Microarrays reveal early transcriptional events during the termination of larval diapause in natural populations of the mosquito, Wyeomyia smithii.
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DOI：
10.1371/journal.pone.0009574
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发表时间：
2010-03-05
期刊：
影响因子：
3.7
通讯作者：
Holzapfel CM
中科院分区：
文献类型：
--
作者：
Emerson KJ;Bradshaw WE;Holzapfel CM
蚊子在幼虫滞育中越冬，幼虫滞育由昼长（光周期）开始、维持和终止。我们使用正向遗传方法来研究长时间暴露后滞育终止所涉及的转录事件。我们采用了一种新颖的方法，比较了两个对单一一天长度有不同反应的人群。我们鉴定了30个与白昼长度差异反应相关的转录本。大多数具有先前注释功能的基因与它们在滞育终止、下游发育事件或从潜在的贫氧环境向富氧环境的转变中所起的作用一致。一个基因从三个独立的前向遗传筛选中脱颖而出，作为光周期定时机制本身（光周期开关）的主要候选基因。我们将该基因命名为光周期反应基因1 （ppdrg1）。WsPpdrg1在长日照响应条件下上调，位于关键光周期QTL上，在极端表型间杂交重组25代后与关键光周期相关。三种独立的正向遗传方法确定WsPpdrg1是一个参与光周期开关机制的基因，或者与光周期开关机制的基因紧密相连。我们的结论是，继续向前的遗传方法不仅对理解光周期和滞育的分子基础至关重要，而且对温带和极地动物种群在面对快速气候变化时的进化潜力也至关重要。
The mosquito Wyeomyia smithii overwinters in a larval diapause that is initiated, maintained and terminated by day length (photoperiod). We use a forward genetic approach to investigate transcriptional events involved in the termination of diapause following exposure to long-days. We incorporate a novel approach that compares two populations that differentially respond to a single day length. We identify 30 transcripts associated with differential response to day length. Most genes with a previously annotated function are consistent with their playing a role in the termination of diapause, in downstream developmental events, or in the transition from potentially oxygen-poor to oxygen-rich environments. One gene emerges from three separate forward genetic screens as a leading candidate for a gene contributing to the photoperiodic timing mechanism itself (photoperiodic switch). We name this gene photoperiodic response gene 1 (ppdrg1). WsPpdrg1 is up-regulated under long-day response conditions, is located under a QTL for critical photoperiod and is associated with critical photoperiod after 25 generations of recombination from a cross between extreme phenotypes. Three independent forward genetic approaches identify WsPpdrg1 as a gene either involved in the photoperiodic switch mechanism or very tightly linked to a gene that is. We conclude that continued forward genetic approaches will be central to understanding not only the molecular basis of photoperiodism and diapause, but also the evolutionary potential of temperate and polar animal populations when confronted with rapid climate change.
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影响因子：
3.3
作者：
BRADSHAW, WE;LOUNIBOS, LP
通讯作者：
LOUNIBOS, LP
影响因子：
2.9
作者：
HARD, JJ;BRADSHAW, WE;HOLZAPFEL, CM
通讯作者：
HOLZAPFEL, CM
影响因子：
3.3
作者：
Bradshaw, WE;Zani, PA;Holzapfel, CM
通讯作者：
Holzapfel, CM
DOI：
10.1007/s00359-008-0334-2
发表时间：
2008-07-01
影响因子：
2.1
作者：
Emerson, Kevin J.;Letaw, Alathea D.;Holzapfel, Christina M.
通讯作者：
Holzapfel, Christina M.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y