Localization and phylogenetic analysis of enzymes related to organellar genome replication in the unicellular rhodophyte Cyanidioschyzon merolae.
Localization and phylogenetic analysis of enzymes related to organellar genome replication in the unicellular rhodophyte Cyanidioschyzon merolae.
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DOI：
10.1093/gbe/evu009
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发表时间：
2014-01
影响因子：
3.3
通讯作者：
Sato N
中科院分区：
文献类型：
--
作者：
Moriyama T;Tajima N;Sekine K;Sato N
植物和藻类具有包含其自身基因组的质体和线粒体，其通过由DNA聚合酶、DNA引发酶、DNA解旋酶、DNA拓扑异构酶、单链DNA维持蛋白、DNA连接酶和引物去除酶组成的装置进行复制。在高等植物拟南芥（Arabidopsis thaliana）中，质体和线粒体中的细胞器复制相关酶（ORE）是相似的，因为它们中的许多都是双重靶向质体和线粒体的。在红藻中，有关于DNA复制酶的报道，植物/原生生物细胞器DNA聚合酶，其定位于质体和线粒体。然而，藻类中的其他ORE仍不清楚。在这里，我们确定了ORE可能定位于单细胞红藻Cyanidioschyzon merolae的细胞器。然后，我们研究了这些酶的绿色荧光蛋白融合蛋白在C. merolae，其细胞具有单个质体和单个双质体，适合于定位分析，表明质体和双质体含有明显不同的复制机制组分。系统发育分析表明，红藻和绿色植物的细胞器复制装置是由各种不同来源的酶组成的，如蛋白细菌、蓝藻和真核生物。特别是在红球藻中，许多蓝藻来源的酶仍然存在。最后，根据定位和系统发育分析的结果，我们提出了真核生物中ORE的继承模型。
Plants and algae possess plastids and mitochondria harboring their own genomes, which are replicated by the apparatus consisting of DNA polymerase, DNA primase, DNA helicase, DNA topoisomerase, single-stranded DNA maintenance protein, DNA ligase, and primer removal enzyme. In the higher plant Arabidopsis thaliana, organellar replication-related enzymes (OREs) are similar in plastids and mitochondria because many of them are dually targeted to plastids and mitochondria. In the red algae, there is a report about a DNA replicase, plant/protist organellar DNA polymerase, which is localized to both plastids and mitochondria. However, other OREs remain unclear in algae. Here, we identified OREs possibly localized to organelles in the unicellular rhodophyte Cyanidioschyzon merolae. We then examined intracellular localization of green fluorescent protein-fusion proteins of these enzymes in C. merolae, whose cell has a single plastid and a single mitochondrion and is suitable for localization analysis, demonstrating that the plastid and the mitochondrion contain markedly different components of replication machinery. Phylogenetic analyses revealed that the organelle replication apparatus was composed of enzymes of various different origins, such as proteobacterial, cyanobacterial, and eukaryotic, in both red algae and green plants. Especially in the red alga, many enzymes of cyanobacterial origin remained. Finally, on the basis of the results of localization and phylogenetic analyses, we propose a model on the succession of OREs in eukaryotes.
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DOI：
10.1016/j.bbagrm.2011.12.005
发表时间：
2012-09
影响因子：
4.7
作者：
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通讯作者：
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影响因子：
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