Micellar electrokinetic chromatography for high-performance analytical separation.
Micellar electrokinetic chromatography for high-performance analytical separation.
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用于高性能分析分离的胶束电动色谱。
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
S. Terabe
中科院分区：
文献类型：
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作者：
S. Terabe
毛细管电泳（CE）是一种较新的分析分离方法，具有分离效率高、样品量少、操作成本低、分离时间快等优点。 CE 是一种分离方法，其中分析物由于分析物上的电荷而在电场下迁移。因此，CE 无法分离中性分析物，直到胶束电动色谱 (MEKC) 出现。 MEKC 是通过向电泳介质中添加离子胶束来进行的，其中一部分分析物被掺入胶束中并具有表观电荷，可以进行电泳分离。 MEKC 中中性分析物的迁移速度取决于分析物的哪一部分掺入胶束中。因此，MEKC 的分离原理与色谱法类似，尽管色谱法中固定相对应的胶束在毛细管内并不是静止的。作者小组对 MEKC 中分析物行为的基本特征和理论处理进行了广泛的研究。 MEKC 已成为 CE 中最流行的分离模式之一。本综述介绍了 MEKC 的开发方式以及它如何作为分析分离方法发挥作用。
Capillary electrophoresis (CE) is a relatively new method of analytical separation having the advantages of high separation efficiency, requirement of a small sample amount, low operating cost, and fast separation time. CE is a separation method where the analyte migrates under an electric field due to a charge on the analyte. Hence, CE was unable to separate neutral analytes until the advent of micellar electrokinetic chromatography (MEKC). MEKC is performed with an addition of ionic micelles to an electrophoretic medium, where a portion of the analyte is incorporated into the micelle and has an apparent charge, which can be subject to electrophoretic separation. The migration velocity of the neutral analyte in MEKC depends on what portion of the analyte is incorporated into the micelle. Thus, the separation principle of MEKC is similar to that of chromatography, although the micelle corresponding to the stationary phase in chromatography is not stationary inside the capillary. The fundamental characteristics and theoretical treatments of the behavior of the analyte in MEKC were studied extensively by the author's group. MEKC has been established as one of the most popular separation modes in CE. This review describes how MEKC was developed and how it is useful as a method of analytical separation.
用于色谱法的两亲性琼脂糖基吸附剂。
DOI：
10.1016/0021-9673(94)00857-6
发表时间：
1995
期刊：
Journal of chromatography. A
影响因子：
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作者：
Oscarsson,S;Angulo-Tatis,D;Chaga,G;Porath,J
通讯作者：
Porath,J