Immunonanoshells for targeted photothermal ablation of tumor cells.
Immunonanoshells for targeted photothermal ablation of tumor cells.
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DOI：
10.2147/nano.2006.1.2.149
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发表时间：
2006
影响因子：
8
通讯作者：
West JL
中科院分区：
文献类型：
--
作者：
Lowery AR;Gobin AM;Day ES;Halas NJ;West JL
纳米壳由薄金壳包围的二氧化硅核组成，具有跨越可见光至近红外（NIR）区域的光学可调谐性，近红外区域是光深入穿透组织的区域。与肿瘤特异性抗体缀合，NIR吸收免疫纳米壳可以优先结合肿瘤细胞。然后近红外光加热结合的纳米壳，从而破坏靶细胞。抗体可以通过双官能聚乙二醇（PEG）接头以每个纳米壳约150个抗体的密度一致地结合到纳米壳。体外研究已经证实了利用免疫纳米壳和NIR光的光热相互作用选择性地诱导细胞死亡的能力。在与抗人表皮生长因子受体（HER 2）免疫纳米壳孵育之前，将表达HER 2的SK-BR-3乳腺癌细胞单独接种或邻近人真皮成纤维细胞（HDF）接种。与HER 2表达细胞结合的抗HER 2免疫纳米壳仅在照射区域内进行近红外暴露后导致SK-BR-3细胞死亡，而HDF在类似处理后仍然存活，因为免疫纳米壳不会与这些细胞高水平结合。与非特异性抗IgG或PEG缀合的对照纳米壳不与任一细胞类型结合，并且细胞在用这些对照纳米壳和NIR照射处理后继续存活。
Consisting of a silica core surrounded by a thin gold shell, nanoshells possess an optical tunability that spans the visible to the near infrared (NIR) region, a region where light penetrates tissues deeply. Conjugated with tumor-specific antibodies, NIR-absorbing immunonanoshells can preferentially bind to tumor cells. NIR light then heats the bound nanoshells, thus destroying the targeted cells. Antibodies can be consistently bound to the nanoshells via a bifunctional polyethylene glycol (PEG) linker at a density of ~150 antibodies per nanoshell. In vitro studies have confirmed the ability to selectively induce cell death with the photothermal interaction of immunonanoshells and NIR light. Prior to incubation with anti-human epidermal growth factor receptor (HER2) immunonanoshells, HER2-expressing SK-BR-3 breast carcinoma cells were seeded alone or adjacent to human dermal fibroblasts (HDFs). Anti-HER2 immunonanoshells bound to HER2-expressing cells resulted in the death of SK-BR-3 cells after NIR exposure only within the irradiated area, while HDFs remained viable after similar treatment since the immunonanoshells did not bind to these cells at high levels. Control nanoshells, conjugated with nonspecific anti-IgG or PEG, did not bind to either cell type, and cells continued to be viable after treatment with these control nanoshells and NIR irradiation.
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DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
6.4
作者：
Verel, I;Heider, KH;van Dongen, GAMS
通讯作者：
van Dongen, GAMS
影响因子：
20.3
作者：
Tarli, L;Balza, E;Zardi, L
通讯作者：
Zardi, L
影响因子：
13.6
作者：
Reynolds, AR;Moghimi, SM;Hodivala-Dilke, K
通讯作者：
Hodivala-Dilke, K
影响因子：
2.8
作者：
Oldenburg, SJ;Averitt, RD;Halas, NJ
通讯作者：
Halas, NJ