Different effects of basic fibroblast growth factor and transforming growth factor-beta on the two platelet-derived growth factor receptors' expression in scleroderma and healthy human dermal fibroblasts.
Different effects of basic fibroblast growth factor and transforming growth factor-beta on the two platelet-derived growth factor receptors' expression in scleroderma and healthy human dermal fibroblasts.
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碱性成纤维细胞生长因子和转化生长因子-β对硬皮病和健康人真皮成纤维细胞中两种血小板源性生长因子受体表达的不同影响。
DOI：
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发表时间：
1995
影响因子：
6.5
通讯作者：
M. Trojanowska
中科院分区：
文献类型：
--
作者：
Yoshiro Ichiki;Edwin A. Smith;E. Leroy;M. Trojanowska
先前的研究表明，硬皮病真皮成纤维细胞中血小板衍生生长因子 (PDGF) α 受体的表达可通过转化生长因子-β 1 (TGF-β 1) 上调，但在健康对照成纤维细胞中则不然。我们通过研究对其他生长因子的反应来询问硬皮病细胞中的这种选择性效应是 TGF-β1 特异性的还是一般反应。在本研究中，我们比较了这两种细胞类型中对碱性成纤维细胞生长因子 (bFGF) 和 TGF-β 1 的反应中 α 和 β PDGF 受体亚基的表达（mRNA 和蛋白质水平）。bFGF 协调刺激健康成纤维细胞中 α 和 β 受体亚基的 mRNA 水平，但不改变硬皮病成纤维细胞中 PDGF 受体的表达。相反，与之前的观察结果一致，TGF-β1 在硬皮病成纤维细胞中诱导 PDGF α 受体表达，但在健康成纤维细胞中则不然。在两种细胞类型中，TGF-β1 诱导 PDGF β 受体 mRNA 水平的程度相似。 PDGF α 受体蛋白水平与 mRNA 水平直接相关，仅在健康成纤维细胞中由 bFGF 诱导，仅在硬皮病成纤维细胞中由 TGF-β 1 诱导。然而，两种细胞类型中的任何生长因子均不会改变 PDGF β 受体蛋白水平。因此，硬皮病成纤维细胞的激活状态不包括bFGF的受体信号传导途径。硬皮病成纤维细胞中 PDGF α 受体的这种独特表达模式表明，协调表达的 PDGF AA 配体/α 受体系统在纤维化发展中可能发挥作用。
Previous studies have demonstrated that platelet-derived growth factor (PDGF) alpha receptor expression is up-regulated by transforming growth factor-beta 1 (TGF-beta 1) in scleroderma dermal fibroblasts, but not in healthy control fibroblasts. We asked whether this selective effect in scleroderma cells was TGF-beta 1-specific or a general response by studying responses to other growth factors. In this study, we compared the expression of alpha and beta PDGF receptor subunits (mRNA and protein levels) in these two cell types in response to basic fibroblast growth factor (bFGF) and TGF-beta 1. bFGF coordinately stimulated mRNA levels of alpha and beta receptor subunits in healthy fibroblasts, but did not change PDGF receptor expression in scleroderma fibroblasts. Conversely, and in agreement with previous observations, TGF-beta 1 induced PDGF alpha receptor expression in scleroderma fibroblasts, but not in healthy fibroblasts. PDGF beta receptor mRNA levels were induced to similar degrees by TGF-beta 1 in both cell types. PDGF alpha receptor protein levels correlated directly with mRNA levels, induced by bFGF only in healthy fibroblasts and by TGF-beta 1 only in scleroderma fibroblasts. However, PDGF beta receptor protein levels were not altered by either growth factor in either cell type. Thus, the activated state of scleroderma fibroblasts does not include receptor-signaling pathways to bFGF. This distinct pattern of expression of PDGF alpha receptors in scleroderma fibroblasts suggests a possible role for the coordinately expressed PDGF AA ligand/alpha receptor system in the development of fibrosis.
DOI：
10.1016/s0021-9258(18)81860-9
发表时间：
1989-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Seifert;C. Hart;P. Phillips;J. Forstrom;R. Ross;M. Murray;D. Bowen-Pope
通讯作者：
R. Seifert;C. Hart;P. Phillips;J. Forstrom;R. Ross;M. Murray;D. Bowen-Pope
通过转化生长因子-β 差异调节两种血小板衍生生长因子受体亚基的表达。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gronwald,RG;Seifert,RA;Bowen-Pope,DF
通讯作者：
Bowen-Pope,DF
DOI：
10.1073/pnas.85.10.3435
发表时间：
1988-05-01
影响因子：
11.1
作者：
GRONWALD, RGK;GRANT, FJ;MURRAY, MJ
通讯作者：
MURRAY, MJ