RNG1 is a late marker of the apical polar ring in Toxoplasma gondii.
RNG1 is a late marker of the apical polar ring in Toxoplasma gondii.
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DOI：
10.1002/cm.20469
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发表时间：
2010-09
期刊：
影响因子：
2.9
通讯作者：
Morrissette, Naomi S.
中科院分区：
文献类型：
--
作者：
Tran, Johnson Q.;de Leon, Jessica C.;Li, Catherine;Huynh, My-Hang;Beatty, Wandy;Morrissette, Naomi S.
顶复门寄生虫的无性繁殖阶段引起诸如疟疾、隐孢子虫病和弓形虫病等疾病的急性症状。这些阶段的特征在于存在两个独立的微管组织中心（MTOC）。中心粒位于核内纺锤体的两极。顶端极环（APR），一个MTOC独特的顶复门，组织膜下微管强加这些原生动物的细胞形状和顶端极性。在这里，我们描述了一种新的蛋白质，定位于我们命名为环-1（RNG 1）的刚地弓形虫的APR的特点。在犬新孢子虫和神经肉孢子虫中存在相关的RNG 1蛋白，但在疟原虫中没有明显的同源物，隐孢子虫属类或巴氏巴氏虫属（Babalypp.）RNG 1是一种小的、低复杂性的、不溶于洗涤剂的蛋白质，其在子寄生虫复制过程的很晚的APR处组装。我们无法敲除RNG 1基因，这表明其基因产物是必需的。标记的RNG 1系也使我们能够在微管破坏药物安磺灵中观察弓形虫生长期间的APR。虽然弓形虫继续生长，但Oryzalin抑制核分裂和胞质分裂，并且与Oryzalin处理的寄生虫中未受抑制的中心粒复制的早期观察相似，APR在异常寄生虫生长期间继续复制。
The asexually proliferating stages of apicomplexan parasites cause acute symptoms of diseases such as malaria, cryptosporidiosis and toxoplasmosis. These stages are characterized by the presence of two independent microtubule organizing centers (MTOCs). Centrioles are found at the poles of the intranuclear spindle. The apical polar ring (APR), a MTOC unique to apicomplexans, organizes subpellicular microtubules which impose cell shape and apical polarity on these protozoa. Here we describe the characteristics of a novel protein that localizes to the APR of Toxoplasma gondii which we have named ring-1 (RNG1). There are related RNG1 proteins in Neospora caninum and Sarcocystis neurona but no obvious homologs in Plasmodium spp., Cryptosporidium spp. or Babesia spp. RNG1 is a small, low-complexity, detergent-insoluble protein that assembles at the APR very late in the process of daughter parasite replication. We were unable to knock-out the RNG1 gene, suggesting that its gene product is essential. Tagged RNG1 lines have also allowed us to visualize the APR during growth of Toxoplasma in the microtubule-disrupting drug oryzalin. Oryzalin inhibits nuclear division and cytokinesis although Toxoplasma growth continues, and similar to earlier observations of unchecked centriole duplication in oryzalin-treated parasites, the APR continues to duplicate during aberrant parasite growth.
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