Nucleotide modifications in three functionally important regions of the Saccharomyces cerevisiae ribosome affect translation accuracy.
Nucleotide modifications in three functionally important regions of the Saccharomyces cerevisiae ribosome affect translation accuracy.
复制标题
DOI：
10.1093/nar/gkp816
复制
发表时间：
2009-12
影响因子：
14.9
通讯作者：
Rousset JP
中科院分区：
文献类型：
--
作者：
Baudin-Baillieu A;Fabret C;Liang XH;Piekna-Przybylska D;Fournier MJ;Rousset JP
RRNA的重要区域富含核苷酸修饰，这些修饰可以对核糖体的生物发生和翻译效率产生强烈的影响。在这里，我们通过删除相应的引导snoRNAs来检测假尿嘧啶和2‘-O-甲基化对酵母翻译准确性的影响。分析的区域是：解码中心(8个修饰)，以及两个亚基间桥域-A位点Finger和Helix 69(6个和5个修饰)。结果表明，一些修饰会影响准确度，影响范围从0.3到2.4倍的野生型活性。阻止修改的子集，尤其是来自解码区的修改子集，会损害停止密码子的终止和阅读帧的维持。出乎意料的是，几个Helix 69突变体拥有更高保真度的核糖体。与强烈的位置效应和协同效应相一致的是，在同一区域，单个缺失可能比多个缺失具有更明显的表型。综上所述，这些结果表明rRNA修饰对翻译准确性有重要作用。
Important regions of rRNA are rich in nucleotide modifications that can have strong effects on ribosome biogenesis and translation efficiency. Here, we examine the influence of pseudouridylation and 2′-O-methylation on translation accuracy in yeast, by deleting the corresponding guide snoRNAs. The regions analyzed were: the decoding centre (eight modifications), and two intersubunit bridge domains—the A-site finger and Helix 69 (six and five modifications). Results show that a number of modifications influence accuracy with effects ranging from 0.3- to 2.4-fold of wild-type activity. Blocking subsets of modifications, especially from the decoding region, impairs stop codon termination and reading frame maintenance. Unexpectedly, several Helix 69 mutants possess ribosomes with increased fidelity. Consistent with strong positional and synergistic effects is the finding that single deletions can have a more pronounced phenotype than multiple deficiencies in the same region. Altogether, the results demonstrate that rRNA modifications have significant roles in translation accuracy.
登录
查看更多内容
影响因子：
64.5
作者：
Brodersen, DE;Clemons, WM;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
影响因子：
14.9
作者：
Davis, DR
通讯作者：
Davis, DR
影响因子：
64.8
作者：
Klaholz, BP;Pape, T;van Heel, M
通讯作者：
van Heel, M
影响因子：
64.8
作者：
Klaholz, BP;Myasnikov, AG;van Heel, M
通讯作者：
van Heel, M
DOI：
10.1073/pnas.0706558104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Ejby, Morten;Sorensen, Michael A.;Pedersen, Steen
通讯作者：
Pedersen, Steen