Interferon‐γ‐inducing Factor Gene Transfection into Lewis Lung Carcinoma Cells Reduces Tumorigenicity in vivo
Interferon‐γ‐inducing Factor Gene Transfection into Lewis Lung Carcinoma Cells Reduces Tumorigenicity in vivo
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干扰素γ诱导因子基因转染Lewis肺癌细胞可降低体内致瘤性
DOI：
10.1111/j.1349-7006.1997.tb00409.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
N. Saijo
中科院分区：
文献类型：
--
作者：
H. Fukumoto;M. Nishio;K. Nishio;Y. Heike;H. Arioka;H. Kurokawa;T. Ishida;K. Fukuoka;T. Nomoto;Y. Ohe;N. Saijo
为了研究鼠干扰素γ诱导因子（mIGIF）的免疫调节作用，我们用含有mIGIF互补DNA的哺乳动物表达载体转染刘易斯肺癌（IXC）细胞。在抗CD 3抗体存在下，转染细胞的培养基刺激脾细胞体外产生干扰素-γ（IFN-γ），并显著增强白细胞介素-12（IL-12）对脾细胞产生IFN-γ的作用。mIGIF转染子细胞显示出致瘤性的降低和对亲本LLC细胞的体内免疫保护作用的诱导。为了检查全身施用重组IL-12（rIL-12）和局部mIGIF对致瘤性的联合作用，在第0天用LLC或转染子细胞攻击小鼠，并从第7天至第11天腹膜内注射50 ng rIL-12。全身性rIL-12显示出抗肿瘤作用。然而，mIGIF基因表达并没有增强全身性rIL-12在体内的这种作用。
To investigate the immunoregulatory effect of murine mterferon‐γ‐inducing factor (mIGIF), we transfected Lewis lung carcinoma (IXC) cells with a mammalian expression vector containing the mIGIF complementary DNA. The culture medium of the transfectant cells stimulated interferon‐γ (IFN‐γ) production by spleen cells in vitro in the presence of anti‐CD3 antibody and markedly potentiated the effect of interleukin‐12 (IL‐12) on IFN‐γ production by spleen cells. mIGIF transfectant cells showed reduction of tumorigenicity and induction of an in vivo immimo‐protective effect against the parental LLC cells. To examine the combined effect of systemic administration of recomhinant IL‐12 (rIL‐12) and local mIGIF on the tumorigenicity, mice were challenged with LLC or transfectant cells on day 0, and the tumor‐bearing mice were injected with 50 ng of rIL‐12 intraperitoneally from day 7 to 11. Systemic rIL‐12 showed an anti‐tumor effect. However, mIGIF gene expression did not potentiate this effect of systemic rIL‐12 in vivo.
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M