Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers.
Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers.
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DOI：
10.1016/j.yexmp.2010.05.002
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发表时间：
2010-10
影响因子：
3.6
通讯作者：
Agrawal, Devendra K.
中科院分区：
文献类型：
--
作者：
Aggarwal, Anshu;Hunter, William J., III;Aggarwal, Himanshu;Silva, Edibaldo D.;Davey, Mary S.;Murphy, Richard F.;Agrawal, Devendra K.
POK家族蛋白不仅在胚胎发育和细胞分化中发挥重要作用，而且在肿瘤发生中也起着重要作用。白血病/淋巴瘤相关因子(LRF)属于POK转录抑制因子家族，也被称为POK红系髓系个体因子(Pokemon)，它与HIV-1(FBI-1)和TTF-1相互作用肽(TIP21)的短转录产物结合。它的致癌作用仅在淋巴瘤、非小细胞肺癌和恶性胶质瘤中已知。LRF在人乳腺癌中的功能表达尚未得到证实。本研究的目的是研究和比较LRF在人类乳腺癌组织和其他人类肿瘤组织中的表达。本文观察了20例乳腺良、恶性活检组织中LRF基因转录产物和蛋白的表达。用免疫组织化学和免疫荧光方法观察到LRF在福尔马林固定的几种组织中的表达。所有恶性乳腺组织均表达LRF的mRNA转录本和蛋白。然而，40%和15%的良性乳腺活检组织分别表达LRF mRNA转录本和蛋白。恶性乳腺组织中LRF mRNA转录本和总蛋白的总表达水平显著高于良性乳腺组织。在人结肠、肾、肺、肝细胞癌和胸腺瘤的细胞核中也观察到LRF的表达。一般来说，LRF在恶性组织中的表达明显高于相应的良性或正常组织。有必要进一步研究以确定LRF在人类乳腺癌中的恶性作用。
The POK family of proteins plays an important role in not only embryonic development and cell differentiation, but also in oncogenesis. Leukemia/lymphoma-related factor (LRF) belongs to the POK family of transcriptional repressors and is also known as POK erythroid myeloid ontogenic factor (POKEMON), which binds to short transcripts of HIV-1 (FBI-1) and TTF-1 interacting peptide (TIP21). Its oncogenic role is known only in lymphoma, non-small cell lung carcinoma, and malignant gliomas. The functional expression of LRF in human breast carcinoma has not yet been confirmed. The aim of this study was to investigate and compare the expression of LRF in human breast cancer tissues and other human tumors. The expression of LRF mRNA transcripts and protein was observed in twenty human benign and malignant breast biopsy tissues. Expression of LRF was observed in several formalin-fixed tissues by immunohistochemistry and immunofluorescence. All malignant breast tissues expressed mRNA transcripts and protein for LRF. However, 40% and 15% benign breast biopsy tissues expressed LRF mRNA transcripts and protein, respectively. The overall expression of LRF mRNA transcripts and total protein was significantly more in malignant breast tissues than the benign breast tissues. LRF expression was also observed in the nuclei of human colon, renal, lung, hepatocellular carcinomas and thymoma tumor cells. In general, a significantly higher expression of LRF was seen in malignant tissues than in the corresponding benign or normal tissue. Further studies are warranted to determine the malignant role of LRF in human breast carcinoma.
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DOI：
10.1074/jbc.m310240200
发表时间：
2004-03-19
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Laudes M;Christodoulides C;Sewter C;Rochford JJ;Considine RV;Sethi JK;Vidal-Puig A;O'Rahilly S
通讯作者：
O'Rahilly S
影响因子：
8
作者：
Davies, JM;Hawe, N;Zelent, A
通讯作者：
Zelent, A
影响因子：
4.8
作者：
Lee, DK;Suh, D;Hur, MW
通讯作者：
Hur, MW
影响因子：
8
作者：
Stogios, Peter J.;Chen, Lu;Prive, Gilbert G.
通讯作者：
Prive, Gilbert G.
影响因子：
8
作者：
Bos, R;van Diest, PJ;Shvarts, A
通讯作者：
Shvarts, A