Overexpression of a putative Arabidopsis BAHD acyltransferase causes dwarfism that can be rescued by brassinosteroid.
Overexpression of a putative Arabidopsis BAHD acyltransferase causes dwarfism that can be rescued by brassinosteroid.
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拟南芥 BAHD 酰基转移酶的过度表达会导致侏儒症，可以通过油菜素类固醇来挽救
DOI：
10.1093/jxb/ers227
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发表时间：
2012-10
影响因子：
6.9
通讯作者：
Yu F
中科院分区：
文献类型：
--
作者：
Wang M;Liu X;Wang R;Li W;Rodermel S;Yu F
植物的生长和发育是通过复杂的管理计划网络来保证的。遗传学方法在剖析这些调控途径方面是非常宝贵的。本研究通过拟南芥T-DNA激活标签突变体筛选，分离到一个半显性矮秆突变体，命名为异常茎1 -1显性（abs 1 -1D）。结果表明，一个新的BAHD家族酰基转移酶基因ABS 1/At 4g 15400的过表达是引起abs 1 -1D矮小表型的原因。ABS 1的过表达导致了许多表型特征，让人想起油菜素类固醇（BR）缺陷或信号突变体，它表明，外源施加BR可以有效地拯救abs 1 -1D的矮化表型。此外，遗传分析表明，abs 1 -1D相互作用，以不同的方式，与BR缺陷型突变体det 2 -1，组成型BR反应突变体bes 1-D和光形态发生突变体phyB-1。此外，ABS 1表达被BR处理或在bes 1-D突变体背景中激活。abs 1 -1D的全基因组转录组分析揭示了代谢途径的明确重编程，并且证明了BR生物合成基因在abs 1 -1D中被激活，并且类黄酮生物合成途径在abs 1 -1D以及det 2 -1中被抑制。这项工作提供了新的见解BAHD酰基转移酶可能参与调节植物生长发育，并表明ABS 1在维持BR稳态的可能作用。
Plant growth and development are ensured through networks of complex regulatory schemes. Genetic approaches have been invaluable in dissecting these regulatory pathways. This study reports the isolation of a semi-dominant dwarf mutant designated abnormal shoot1-1 dominant (abs1-1D) through an Arabidopsis T-DNA activation tagging mutant screen. It was shown that the overexpression of a novel BAHD family acyltransferase gene, ABS1/At4g15400, was the cause of the dwarf phenotype in abs1-1D. Overexpression of ABS1 led to many phenotypic features reminiscent of brassinosteroid (BR) deficient or signalling mutants, and it was shown that exogenously applied BR could effectively rescue the dwarf phenotype of abs1-1D. Furthermore, genetic analyses indicated that abs1-1D interacted, in different ways, with the BR-deficient mutant det2-1, the constitutive BR response mutant bes1-D and the photomorphogenic mutant phyB-1. Moreover, ABS1 expression was activated by BR treatment or in a bes1-D mutant background. Genome-wide transcriptome profiling of abs1-1D revealed clear reprogramming of metabolic pathways, and it was demonstrated that BR biosynthesis genes were activated in abs1-1D and that the flavonoid biosynthesis pathway was repressed in abs1-1D, as well as in det2-1. This work provides new insights into the possible involvement of BAHD acyltransferase in the regulation of plant growth and development, and indicates a possible role of ABS1 in maintaining BR homeostasis.
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影响因子：
7.4
作者：
Goda, H;Shimada, Y;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
64.8
作者：
Peng, JR;Richards, DE;Harberd, NP
通讯作者：
Harberd, NP
影响因子：
4.8
作者：
Ma, XY;Koepke, J;Stöckigt, J
通讯作者：
Stöckigt, J
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
6.9
作者：
Nakamura, M;Satoh, T;Nagatani, A
通讯作者：
Nagatani, A