Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry.
Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry.
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细胞内蛋白质寡聚物化学计量定量的单分子显微镜。
DOI：
10.1016/j.sbi.2020.10.022
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发表时间：
2021-03
影响因子：
6.8
通讯作者：
Chen TY
中科院分区：
文献类型：
--
作者：
Chen H;Xie X;Chen TY
Protein organization modification plays a vital role in initiating signaling pathways, transcriptional regulation, and cell apoptosis regulation. Simultaneous quantification of oligomeric state and cellular parameters in the same cell, even though challenging, is required to understand their correlation at the molecular level. Recent advances of fluorescence protein and single-molecule localization microscopy enables the determination of localizations and oligomeric states of target proteins in cells. We reviewed the fluorescence intensity-based, localization-based, and photophysical property-based approaches for in-cell quantification of protein oligomeric stoichiometry. We discussed their working principles, applications, advantages, and limitations. These results also imply the combination of methodologies targeting different biological parameters at the single-cell level is essential to uncover the structure-function relationship at the molecular level.
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影响因子：
16.6
作者：
Chen TY;Santiago AG;Jung W;Krzemiński Ł;Yang F;Martell DJ;Helmann JD;Chen P
通讯作者：
Chen P
影响因子：
16.6
作者：
de Castro, Maria Angela Gomes;Wildhagen, Hanna;Opazo, Felipe
通讯作者：
Opazo, Felipe
单分子成像显示活细胞中 CXCR4 寡聚状态的配体调节
影响因子：
3.3
作者：
Lao, Jun;He, Hua;Huang, Fang
通讯作者：
Huang, Fang
影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC
影响因子：
3.4
作者：
Baldering, Tim N.;Bullerjahn, Jakob T.;Malkusch, Sebastian
通讯作者：
Malkusch, Sebastian