Microscale 2D separation systems for proteomic analysis.
Microscale 2D separation systems for proteomic analysis.
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DOI：
10.1586/epr.12.10
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发表时间：
2012-04
影响因子：
3.4
通讯作者：
Fan ZH
中科院分区：
文献类型：
--
作者：
Xu X;Liu K;Fan ZH
微尺度的2D分离系统已经在毛细管和微制造通道中实现。它们具有分析速度快、分离效率高、样品消耗少等优点，与传统的柱中液相色谱(LC)和平板凝胶电泳法相比具有明显的优势。本文综述了近几年来毛细管电泳法的研究进展，重点介绍了2维毛细管电泳法、毛细管电泳联用毛细管电泳法和微流控技术。通过巧妙设计的界面，采用了多种CE和LC模式来构建2D分离系统。在许多微流控装置中也实现了不同分离模式的耦合。这些分离系统已被应用于从单个细胞到肿瘤组织的各种生物样本的蛋白质组学分析。
Microscale 2D separation systems have been implemented in capillaries and microfabricated channels. They offer advantages of faster analysis, higher separation efficiency and less sample consumption than the conventional methods, such as liquid chromatography (LC) in a column and slab gel electrophoresis. In this article, we review their recent advancement, focusing on three types of platforms, including 2D capillary electrophoresis (CE), CE coupling with capillary LC, and microfluidic devices. A variety of CE and LC modes have been employed to construct 2D separation systems via sophistically designed interfaces. Coupling of different separation modes has also been realized in a number of microfluidic devices. These separation systems have been applied for the proteomic analysis of various biological samples, ranging from a single cell to tumor tissues.
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影响因子：
2.9
作者：
Dickerson, Jane A.;Ramsay, Lauren M.;Dada, Oluwatosin O.;Cermak, Nathan;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
影响因子：
7.4
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影响因子：
7.4
作者：
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通讯作者：
Lee, CS
影响因子：
4.2
作者：
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通讯作者：
Bergquist, Jonas
影响因子：
4.2
作者：
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通讯作者：
Zenobi, Renato