Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature's perfect packages.
Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature's perfect packages.
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DOI：
10.1016/j.jbc.2022.102483
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发表时间：
2022-10
影响因子：
4.8
通讯作者：
Gaborski, Thomas
中科院分区：
文献类型：
--
作者：
Michel, Lea Vacca;Gaborski, Thomas
败血症通常是对感染的一种危及生命的反应，发生在宿主的促炎免疫反应异常升高和失调时。要诊断败血症，患者必须有确诊或预测的感染，以及与败血症病理生理相关的其他症状。然而，最近的一项研究发现，在大多数（70.1%）败血症病例中，无法确定特定的致病微生物，可能是由于侵袭性抗生素或局部感染。患者败血症诊断的时间通常可以预测其临床结果，强调需要更明确的分子诊断测试。在这里，我们概述了利用细菌外膜囊泡（OMVs）作为诊断革兰氏阴性脓毒症的生物标志物的优势和挑战。细菌外膜囊泡是来自革兰氏阴性细菌外膜的纳米级球形芽。优势包括OMV丰度，抗生素存在下的稳健性，以及它们源自亲本细胞的独特特征，可以允许细菌物种之间的分化。挑战包括从复杂生物流体中分离omv所需的严格纯化方法，以及将omv从与omv具有相同物理特性的类似大小的细胞外囊泡中分离的额外需求。
Sepsis is an often life-threatening response to infection, occurring when host proinflammatory immune responses become abnormally elevated and dysregulated. To diagnose sepsis, the patient must have a confirmed or predicted infection, as well as other symptoms associated with the pathophysiology of sepsis. However, a recent study found that a specific causal organism could not be determined in the majority (70.1%) of sepsis cases, likely due to aggressive antibiotics or localized infections. The timing of a patient’s sepsis diagnosis is often predictive of their clinical outcome, underlining the need for a more definitive molecular diagnostic test. Here, we outline the advantages and challenges to using bacterial outer membrane vesicles (OMVs), nanoscale spherical buds derived from the outer membrane of Gram-negative bacteria, as a diagnostic biomarker for Gram-negative sepsis. Advantages include OMV abundance, their robustness in the presence of antibiotics, and their unique features derived from their parent cell that could allow for differentiation between bacterial species. Challenges include the rigorous purification methods required to isolate OMVs from complex biofluids and the additional need to separate OMVs from similarly sized extracellular vesicles, which can share physical properties with OMVs.
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幸存的败血症运动：严重败血症和化粪池冲击管理的国际指南，2012年。
影响因子：
38.9
作者：
Dellinger RP;Levy MM;Rhodes A;Annane D;Gerlach H;Opal SM;Sevransky JE;Sprung CL;Douglas IS;Jaeschke R;Osborn TM;Nunnally ME;Townsend SR;Reinhart K;Kleinpell RM;Angus DC;Deutschman CS;Machado FR;Rubenfeld GD;Webb S;Beale RJ;Vincent JL;Moreno R;Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup
通讯作者：
Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup
DOI：
10.1038/nrmicro3480
发表时间：
2015-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者：
Casadevall A
影响因子：
2.3
作者：
Arabi Y;Alamry A;Levy MM;Taher S;Marini AM
通讯作者：
Marini AM
影响因子：
4.6
作者：
Brennan, K.;Martin, K.;Mc Gee, M. M.
通讯作者：
Mc Gee, M. M.
败血症的诊断和处理
影响因子：
4.4
作者：
Evans, Tom
通讯作者：
Evans, Tom