A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions
A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions
复制标题
一种基于文库的方法，可快速分析多个基因组区域的染色质可及性
作者：
A. Basheer;Harald Berger;Y. Reyes;M. Gorfer;J. Strauss
传统的染色质分析方法一次只能检测一个基因座，或者对每个基因座使用不同的模板，这使得对不同基因座的大基因组区域或许多共调控基因进行标准化分析成为一项困难的任务。另一方面，采用大规模并行测序平台对染色质可及性进行全基因组高分辨率绘图会产生大量分析起来很费力的数据集，并且是一种成本密集型方法，仅适用于分析有限的生物样本集。为了缩小传统和高通量程序之间的差距，我们开发了一种方法，其中产生条件特异性的全基因组染色质片段文库，然后用于基因座特异性DNA片段分析。为了验证该方法，我们使用，作为一个测试位点，研究启动子的分歧转录niaA和niaD基因编码的硝酸盐同化酶曲霉。此外，我们已经使用条件特异性文库来研究核小体定位在两个不同的位点，一般氮调节剂的启动子areA和次级代谢的调节剂aflR。
Traditional chromatin analysis methods only test one locus at the time or use different templates for each locus, making a standardized analysis of large genomic regions or many co-regulated genes at different loci a difficult task. On the other hand, genome-wide high-resolution mapping of chromatin accessibility employing massive parallel sequencing platforms generates an extensive data set laborious to analyse and is a cost-intensive method, only applicable to the analysis of a limited set of biological samples. To close this gap between the traditional and the high-throughput procedures we have developed a method in which a condition-specific, genome-wide chromatin fragment library is produced and then used for locus-specific DNA fragment analysis. To validate the method, we used, as a test locus, the well-studied promoter of the divergently transcribed niiA and niaD genes coding for nitrate assimilation enzymes in Aspergillus. Additionally, we have used the condition-specific libraries to study nucleosomal positioning at two different loci, the promoters of the general nitrogen regulator areA and the regulator of secondary metabolism, aflR.
影响因子：
4
作者：
Berger, SL
通讯作者：
Berger, SL