Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.
Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.
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DOI：
10.1091/mbc.e21-05-0239
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发表时间：
2021-08-01
影响因子：
3.3
通讯作者：
Jaspersen SL
中科院分区：
文献类型：
--
作者：
Bestul AJ;Yu Z;Unruh JR;Jaspersen SL
粟酒裂殖酵母（Schizosaccharomyces pombe）中适当的有丝分裂进程需要部分核膜崩解（NEBD）以及纺锤体极体（SPB——酵母中心体）的插入以构建有丝分裂纺锤体。着丝粒与SPB的连接对此过程至关重要，但其重要性的原因尚未完全明了。利用高分辨率结构照明显微镜，我们发现保守的Sad1 - UNC - 84同源结构域蛋白Sad1和其他SPB蛋白在有丝分裂期间重新分布，在SPB周围形成一个环复合物，这是局部NEBD和纺锤体形成的前体。尽管Polo激酶Plo1对Sad1的重新分布不是必需的，但它定位于与着丝粒相连的SPB区域，并且其活性对其他SPB环蛋白的重新分布以及SPB处的完全NEBD（以允许SPB插入）至关重要。我们的结果引出了一个模型，即着丝粒与SPB的连接驱动Sad1的重新分布和Plo1的激活，进而通过构建SPB环结构促进部分NEBD和纺锤体形成。
Proper mitotic progression in Schizosaccharomyces pombe requires partial nuclear envelope breakdown (NEBD) and insertion of the spindle pole body (SPB—yeast centrosome) to build the mitotic spindle. Linkage of the centromere to the SPB is vital to this process, but why that linkage is important is not well understood. Utilizing high-resolution structured illumination microscopy, we show that the conserved Sad1-UNC-84 homology-domain protein Sad1 and other SPB proteins redistribute during mitosis to form a ring complex around SPBs, which is a precursor for localized NEBD and spindle formation. Although the Polo kinase Plo1 is not necessary for Sad1 redistribution, it localizes to the SPB region connected to the centromere, and its activity is vital for redistribution of other SPB ring proteins and for complete NEBD at the SPB to allow for SPB insertion. Our results lead to a model in which centromere linkage to the SPB drives redistribution of Sad1 and Plo1 activation that in turn facilitate partial NEBD and spindle formation through building of a SPB ring structure.
coper牛豆状真菌乌莫罗司莫尔斯叶片中有丝分裂的超微结构。 vignae。
影响因子：
7.8
作者：
Heath, I B;Heath, M C
通讯作者：
Heath, M C