Xanthomonas hydrangeae sp. nov., a novel plant pathogen isolated from Hydrangea arborescens.
Xanthomonas hydrangeae sp. nov., a novel plant pathogen isolated from Hydrangea arborescens.
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绣球黄单胞菌
DOI：
10.1099/ijsem.0.005163
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发表时间：
2021
影响因子：
2.8
通讯作者：
J. Pothier
中科院分区：
文献类型：
--
作者：
Nay C. Dia;J. Van Vaerenbergh;C. Van Malderghem;J. Blom;T. H. Smits;B. Cottyn;J. Pothier
本文描述了2011年和2012年从比利时弗兰德斯苗圃种植的绣球品种中分离出的一个新物种。在28 °C下4天后，菌株产生黄色、圆形、凸形和粘液样菌落。菌株的致病性，证实其分离宿主，以及对绣球。使用MALDI-TOF MS的分析将绣球属菌株鉴定为属于黄单胞菌属，但将它们从植物黄单胞菌物种中排除。基于gyrB的系统发育树证实了该菌与X. hortorum。在绣球属分离物中占优势的有三种脂肪酸：anteiso-C15：0、iso-C15：0和总特征3（C16：1 ω 7 c/C16：1 ω6c）。 不像X。对于hortorum致病变种，八仙花菌株不能在氯化锂存在下生长，并且只能微弱地利用d-果糖-6-PO 4和葡糖醛酸酰胺。基于多位点序列分析的系统发育特征和基因组特征表明，菌株接近，但不同于X。hortorum。各菌株的基因组序列与X. 禾本科致病变种建立了一种基于基因组学的环介导等温扩增方法，该方法对八仙花属菌株具有特异性，可用于早期检测。报道了一新种，绣球黄单胞菌Xanthomonashydrangeaesp. nov.，以LMG 31884 T（=CCOS 1956 T）菌株为模式菌株。
This paper describes a novel species isolated in 2011 and 2012 from nursery-grown Hydrangea arborescens cultivars in Flanders, Belgium. After 4 days at 28 °C, the strains yielded yellow, round, convex and mucoid colonies. Pathogenicity of the strains was confirmed on its isolation host, as well as on Hydrangea quercifolia. Analysis using MALDI-TOF MS identified the Hydrangea strains as belonging to the genus Xanthomonas but excluded them from the species Xanthomonas hortorum. A phylogenetic tree based on gyrB confirmed the close relation to X. hortorum. Three fatty acids were dominant in the Hydrangea isolates: anteiso-C15 : 0, iso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c). Unlike X. hortorum pathovars, the Hydrangea strains were unable to grow in the presence of lithium chloride and could only weakly utilize d-fructose-6-PO4 and glucuronamide. Phylogenetic characterization based on multilocus sequence analysis and phylogenomic characterization revealed that the strains are close to, yet distinct from, X. hortorum. The genome sequences of the strains had average nucleotide identity values ranging from 94.35-95.19 % and in silico DNA-DNA hybridization values ranging from 55.70 to 59.40 % to genomes of the X. hortorum pathovars. A genomics-based loop-mediated isothermal amplification assay was developed which was specific to the Hydrangea strains for its early detection. A novel species, Xanthomonas hydrangeae sp. nov., is proposed with strain LMG 31884T (=CCOS 1956T) as the type strain.
对90K核基因组的高通量ANI分析揭示了清晰的物种边界。
影响因子：
16.6
作者：
Jain C;Rodriguez-R LM;Phillippy AM;Konstantinidis KT;Aluru S
通讯作者：
Aluru S