A 7 gene expression score predicts for radiation response in cancer cervix.
A 7 gene expression score predicts for radiation response in cancer cervix.
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7 基因表达评分可预测宫颈癌的放射反应。
DOI：
10.1186/1471-2407-9-365
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发表时间：
2009-10-15
期刊：
影响因子：
3.8
通讯作者：
Nambaru L
中科院分区：
文献类型：
--
作者：
Rajkumar T;Vijayalakshmi N;Sabitha K;Shirley S;Selvaluxmy G;Bose MV;Nambaru L
宫颈癌是印度妇女中最常见的癌症。目前的建议是治疗IIB，IIIA，IIIB和IVA期根治性放疗和每周顺铂为基础的化疗。然而，单独的放射治疗可以帮助治愈超过60%的IIB期患者和高达40%的IIIB期患者。来自15名达到完全缓解并保持无病超过36个月的患者（无疾病证据或NED组）和10名根治性放疗失败的患者（失败组）的存档RNA样本被纳入研究。将RNA扩增、标记并与斯坦福大学微阵列芯片杂交，并使用BRB Array Tools软件和微阵列显著性分析（SAM）分析进行分析。选择20个基因用于以Taqman低密度阵列（TLDA）格式使用相对定量（RQ）Taqman测定法进行进一步验证。使用每个NED样本一次作为校准品，计算RQ值。基于基因的RQ值开发了评分系统。使用基于七个基因的评分系统，可以区分可能对放射治疗有反应的肿瘤和可能失败的肿瘤。以平均值± 2 SE（标准误）为标准，以5.6 0为界值，低危组的敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV）分别为0.6 4、1.0、1.0和0.6 7。我们已经确定了一个7基因签名，可以帮助识别可以单独接受放射治疗的宫颈癌患者。然而，这需要在更大的患者人群中进行验证。
Cervical cancer is the most common cancer among Indian women. The current recommendations are to treat the stage IIB, IIIA, IIIB and IVA with radical radiotherapy and weekly cisplatin based chemotherapy. However, Radiotherapy alone can help cure more than 60% of stage IIB and up to 40% of stage IIIB patients. Archival RNA samples from 15 patients who had achieved complete remission and stayed disease free for more than 36 months (No Evidence of Disease or NED group) and 10 patients who had failed radical radiotherapy (Failed group) were included in the study. The RNA were amplified, labelled and hybridized to Stanford microarray chips and analyzed using BRB Array Tools software and Significance Analysis of Microarray (SAM) analysis. 20 genes were selected for further validation using Relative Quantitation (RQ) Taqman assay in a Taqman Low-Density Array (TLDA) format. The RQ value was calculated, using each of the NED sample once as a calibrator. A scoring system was developed based on the RQ value for the genes. Using a seven gene based scoring system, it was possible to distinguish between the tumours which were likely to respond to the radiotherapy and those likely to fail. The mean score ± 2 SE (standard error of mean) was used and at a cut-off score of greater than 5.60, the sensitivity, specificity, Positive predictive value (PPV) and Negative predictive value (NPV) were 0.64, 1.0, 1.0, 0.67, respectively, for the low risk group. We have identified a 7 gene signature which could help identify patients with cervical cancer who can be treated with radiotherapy alone. However, this needs to be validated in a larger patient population.
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影响因子：
6
作者：
Lu, ML;Nakamura, RM;Tan, EM
通讯作者：
Tan, EM
影响因子：
5.7
作者：
Kim, Tae-Joong;Choi, Jung-Joo;Kim, Byoung-Gie
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影响因子：
3.8
作者：
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通讯作者：
Jezequel, Pascal
影响因子：
11.2
作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
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