Measuring Skeletal Muscle Thermogenesis in Mice and Rats.
Measuring Skeletal Muscle Thermogenesis in Mice and Rats.
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DOI：
10.3791/64264
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发表时间：
2022-07-27
期刊：
影响因子：
--
通讯作者：
Novak CM
中科院分区：
文献类型：
--
作者：
Watts CA;Haupt A;Smith J;Welch E;Malik A;Giacomino R;Walter D;Mavundza N;Shemery A;Caldwell HK;Novak CM
骨骼肌产热为更好地理解代谢动态平衡和潜在的能量消耗机制提供了潜在的途径。令人惊讶的是，几乎没有证据可以将生热的神经、肌细胞和分子机制与肌肉温度的可测量变化直接联系起来。本文描述了一种利用温度应答器恢复小鼠和大鼠骨骼肌温度的直接测量的方法。通过手术将远程应答器植入小鼠和大鼠的肌肉内，让动物有时间恢复。然后，小鼠和大鼠必须反复适应测试环境和程序。肌肉温度的变化是在家庭笼子里对药物或背景刺激做出反应而测量的。也可以在规定的身体活动期间测量肌肉温度(即，以恒定速度在跑步机上行走)，以排除活动中的变化是这些刺激引起的肌肉温度变化的因素。这种方法已经成功地用于在大脑、交感神经系统和骨骼肌水平上阐明肌肉产热控制的机制。提供了使用捕食者气味(PO；雪貂气味)作为上下文刺激和注射催产素(OXT)作为药理刺激的这种成功的演示，其中捕食者气味诱导肌肉产热，OXT抑制肌肉温度。因此，这些数据集显示了该方法在检测肌肉温度快速变化方面的有效性。
Skeletal muscle thermogenesis provides a potential avenue for better understanding metabolic homeostasis and mechanisms underlying energy expenditure. Surprisingly little evidence is available to link neural, myocellular, and molecular mechanisms of thermogenesis directly to measurable changes in muscle temperature. This paper describes a method in which temperature transponders are utilized to retrieve direct measurements of mouse and rat skeletal muscle temperature. Remote transponders are surgically implanted within the muscle of mice and rats, and the animals are given time to recover. Mice and rats must then be repeatedly habituated to the testing environment and procedure. Changes in muscle temperature are measured in response to pharmacological or contextual stimuli in the home cage. Muscle temperature can also be measured during prescribed physical activity (i.e., treadmill walking at a constant speed) to factor out changes in activity as contributors to the changes in muscle temperature induced by these stimuli. This method has been successfully used to elucidate mechanisms underlying muscle thermogenic control at the level of the brain, sympathetic nervous system, and skeletal muscle. Provided are demonstrations of this success using predator odor (PO; ferret odor) as a contextual stimulus and injections of oxytocin (Oxt) as a pharmacological stimulus, where predator odor induces muscle thermogenesis, and Oxt suppresses muscle temperature. Thus, these datasets display the efficacy of this method in detecting rapid changes in muscle temperature.
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