Inhibitors of topoisomerases do not block the passage of human lymphocyte chromosomes through mitosis.
Inhibitors of topoisomerases do not block the passage of human lymphocyte chromosomes through mitosis.
复制标题
拓扑异构酶抑制剂不会阻止人淋巴细胞染色体通过有丝分裂。
作者：
A. T. Sumner
培养的人淋巴细胞已被处理与一些拓扑异构酶抑制剂，看看拓扑异构酶II是否参与染色体分离的过程中在后期。通过检查扩散染色体的细胞遗传学制备物来评估结果。观察到四种效应，尽管没有抑制剂产生所有四种效应。这些影响是：抑制进入有丝分裂;染色体断裂和重排;抑制染色体凝聚;和抑制染色体分离。最后一个证据是模棱两可的。尽管有证据表明，当细胞用秋水仙碱以及拓扑异构酶II抑制剂（最显著的是萘二甲酸，其导致染色单体的完全融合）处理时，染色单体的分离受到影响，但没有证据表明，在没有秋水仙碱的情况下，用抑制剂处理的细胞不能正常地进行后期。拓扑异构酶I抑制剂喜树碱与拓扑异构酶II抑制剂的不同之处在于对染色体凝聚没有任何影响或对分离没有任何显著影响。
Cultured human lymphocytes have been treated with a number of topoisomerase inhibitors, to see whether topoisomerase II is involved in the process of chromosome segregation at anaphase. Results were assessed by examination of cytogenetical preparations of spread chromosomes. Four effects were observed, although no inhibitor produced all four effects. These effects were: inhibition of entry into mitosis; chromosome breakage and rearrangement; inhibition of chromosome condensation; and inhibition of chromosome segregation. Evidence for the last was ambiguous. Although there was evidence that separation of chromatids was affected when cells were treated with colchicine as well as topoisomerase II inhibitors (most notably with nalidixic acid, which resulted in complete fusion of the chromatids), no evidence was obtained to show that, in the absence of colchicine, cells treated with inhibitors could not proceed through anaphase normally. The topoisomerase I inhibitor, camptothecin, differed from the topoisomerase II inhibitors in not showing any effect on chromosome condensation or any significant effect on segregation.
新生霉素、萘啶酸、依托泊苷和 4-(9-吖啶基氨基)甲磺酰-甲氧基苯胺对 G2 期和有丝分裂中国仓鼠卵巢细胞进展的影响。
影响因子：
11.2
作者：
Rowley,R;Kort,L
通讯作者：
Kort,L
DOI：
10.1073/pnas.81.9.2616
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DINARDO, S;VOELKEL, K;STERNGLANZ, R
通讯作者：
STERNGLANZ, R
影响因子：
56.9
作者：
KOSHLAND, D;HARTWELL, LH
通讯作者：
HARTWELL, LH