The Genetic Network of Forkhead Gene Family in Development of Brown Planthoppers.
The Genetic Network of Forkhead Gene Family in Development of Brown Planthoppers.
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褐飞虱发育过程中叉头基因家族的遗传网络
DOI：
10.3390/biology10090867
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发表时间：
2021-09-03
期刊：
影响因子：
4.2
通讯作者：
Ye YX
中科院分区：
文献类型：
--
作者：
Lin HY;Zhu CQ;Zhang HH;Shen ZC;Zhang CX;Ye YX
Forkhead（Fox）基因编码一个转录因子家族，该家族由一个“翼螺旋”DNA结合结构域定义，在调节细胞生长、增殖、分化和寿命相关基因的表达中起重要作用。然而，我们仍然缺乏对动物中Fox基因家族的全面了解。本研究采用基因组学、转录组学和表型组学相结合的方法，构建了水稻主要害虫褐飞虱Fox基因的遗传网络。我们发现，FoxG，FoxQ，FoxA，FoxN 1，FoxN 2及其潜在的靶基因是胚胎发生不可缺少的; FoxC，FoxJ 1和FoxP具有互补作用的晚期胚胎发生; FoxA，FoxN 1和FoxQ是多效性，也是必要的若虫蜕皮; FoxT属于一个新的昆虫特异性狐狸亚科;和FoxL 2和FoxO分别参与蛋壳和翅膀的发展。这些发现可能会加深我们对Fox基因家族在昆虫生长发育中的调控功能的整体理解，从而最终刺激新型杀虫剂的设计和开发。我们在褐飞虱基因组中鉴定了18个不同的Fox基因，并进一步发现了一个新的昆虫特异性亚家族，我们暂时命名为FoxT。共有16个基因在卵子中高度表达，其中NlFoxL 2和NlFoxT分别是雌性和雄性特异性基因。使用大规模RNAi和RNA-seq分析来揭示NlFoxs的功能和潜在靶点。在卵中，NlFoxA、NlFoxN 1和NlFoxN 2通过调节不同的靶基因而对早期胚胎发生不可或缺; NlFoxG和NlFoxQ共调节NlSix 3以促进脑发育; NlFoxC、NlFoxJ 1和NlFoxP对晚期胚胎发生具有互补作用。此外，NlFoxA、NlFoxNl和NlFoxQ具有多效性。NlFoxA和NlFoxQ分别调节NlCHS 1和表皮蛋白的表达，从而参与表皮的形成。调节NlKrt 9表达的NlFoxNl参与中间丝框架的形成。我们以前的研究表明，NlFoxL 2和NlFoxO在绒毛膜形成和翅多型性中起重要作用。总之，N。Fox基因在胚胎发育和器官发生中表现出功能多样性。这项综合研究结合了基因组学、转录组学和表型组学，从而构建了一个复杂的遗传网络，涵盖了褐飞虱的整个生命周期。
Forkhead (Fox) genes encode a family of transcription factors defined by a ‘winged helix’ DNA-binding domain and play important roles in regulating the expression of genes involved in cell growth, proliferation, differentiation and longevity. However, we still lack a comprehensive understanding of the Fox gene family in animals. Here, we take an integrated study, which combines genomics, transcriptomics and phenomics, to construct the Fox gene genetic network in the brown planthopper, Nilaparvata lugens, a major rice pest. We show that FoxG, FoxQ, FoxA, FoxN1, FoxN2 and their potential target genes are indispensable for embryogenesis; FoxC, FoxJ1 and FoxP have complementary effects on late embryogenesis; FoxA, FoxNl and FoxQ are pleiotropism and also essential for nymph molting; FoxT belongs to a novel insect-specific Fox subfamily; and FoxL2 and FoxO are involved in the development of eggshells and wings, respectively. These findings may deepen our overall understanding of the regulatory function of the Fox gene family in insect growth and development, and thus ultimately stimulate the design and development of novel insecticides. We identified 18 distinct Fox genes in the genome of the brown planthopper, Nilaparvata lugens, and further found a novel insect-specific subfamily that we temporarily named FoxT. A total of 16 genes were highly expressed in the eggs, while NlFoxL2 and NlFoxT are female- and male-specific genes, respectively. Large scale RNAi and RNA-seq analyses were used to reveal the functions and potential targets of NlFoxs. In the eggs, NlFoxA, NlFoxN1 and NlFoxN2 are indispensable to early embryogenesis by regulating different target genes; NlFoxG and NlFoxQ co-regulate NlSix3 for brain development; and NlFoxC, NlFoxJ1 and NlFoxP have complementary effects on late embryogenesis. Moreover, NlFoxA, NlFoxNl and NlFoxQ have pleiotropism. NlFoxA and NlFoxQ regulate the expression of NlCHS1 and cuticular proteins, respectively, thereby participating in the formation of cuticles. NlFoxN1, which regulates the expression of NlKrt9 is involved in the formation of intermediate filament frameworks. Our previous studies have revealed that NlFoxL2 and NlFoxO play important roles in chorion formation and wing polyphenism. Altogether, N. lugens Fox genes exhibit functional diversity in embryonic development and organogenesis. This comprehensive study combines genomics, transcriptomics and phenomics, thereby constructing a complex genetic network that spans the entire life cycle of the brown planthopper.
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影响因子：
4.5
作者：
Posnien N;Koniszewski ND;Hein HJ;Bucher G
通讯作者：
Bucher G
DOI：
10.1073/pnas.89.18.8754
发表时间：
1992-09-15
影响因子：
11.1
作者：
HACKER, U;GROSSNIKLAUS, U;JACKLE, H
通讯作者：
JACKLE, H
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
3.8
作者：
Hamodrakas, SJ;Willis, JH;Iconomidou, VA
通讯作者：
Iconomidou, VA