Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber.
Tuning Shear Thinning Factors of 3D Bio-Printable Hydrogels Using Short Fiber.
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DOI：
10.3390/ma16020572
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发表时间：
2023-01-06
期刊：
影响因子：
3.4
通讯作者：
Habib, Ahasan
中科院分区：
文献类型：
--
作者：
Tuladhar, Slesha;Clark, Scott;Habib, Ahasan
在各种可用的 3D 生物打印技术中，基于挤出的三维 (3D) 生物打印允许沉积充满细胞的生物墨水，确保预定义的支架结构可提供活组织再生。天然水凝胶具有生物相容性、细胞毒性低和含水量高等独特特性，是基于挤出的 3D 生物打印工艺的生物墨水配方的绝佳候选者。然而，由于其机械完整性较低，水凝胶在保持结构完整性方面面临着共同的挑战。为了应对这一挑战，我们探索了由纤维素衍生的纳米纤维（TEMPO介导的纳米原纤化纤维素，TO-NFC）、羧甲基纤维素（CMC）和常用藻酸盐组成的一组混合水凝胶的流变特性，特别是剪切稀化行为（随着水凝胶上施加的载荷/剪切速率的增加而降低粘度）。使用较高百分比（0.5％和1.0％）和较低百分比（0.005％和0.01％）的TO-NFC、1-4％的CMC和1-4％的藻酸盐制备了总共46种组合物，以分析剪切稀化因子，例如n和K值，这些因子是根据流程图确定的，并与3D打印适性相关。通过不同比例的纳米纤维调节剪切稀化因子的能力有助于实现具有明确支架结构的 3D 生物打印支架。
Among various available 3D bioprinting techniques, extrusion-based three-dimensional (3D) bioprinting allows the deposition of cell-laden bioink, ensuring predefined scaffold architecture that may offer living tissue regeneration. With a combination of unique characteristics such as biocompatibility, less cell toxicity, and high water content, natural hydrogels are a great candidate for bioink formulation for the extrusion-based 3D bioprinting process. However, due to its low mechanical integrity, hydrogel faces a common challenge in maintaining structural integrity. To tackle this challenge, the rheological properties, specifically the shear thinning behavior (reduction of viscosity with increasing the applied load/shear rate on hydrogels) of a set of hybrid hydrogels composed of cellulose-derived nanofiber (TEMPO-mediated nano-fibrillated cellulose, TO-NFC), carboxymethyl cellulose (CMC), and commonly used alginate, were explored. A total of 46 compositions were prepared using higher (0.5% and 1.0%) and lower percentages (0.005% and 0.01%) of TO-NFC, 1–4% of CMC, and 1–4% of alginate to analyze the shear thinning factors such as the values of n and K, which were determined for each composition from the flow diagram and co-related with the 3D printability. The ability to tune shear thinning factors with various ratios of a nanofiber can help achieve a 3D bio-printed scaffold with defined scaffold architecture.
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