Galectin-7 (PIG1) Exhibits Pro-apoptotic Function through JNK Activation and Mitochondrial Cytochrome cRelease* 210
Galectin-7 (PIG1) Exhibits Pro-apoptotic Function through JNK Activation and Mitochondrial Cytochrome cRelease* 210
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Galectin-7 (PIG1) 通过 JNK 激活和线粒体细胞色素 cRelease 表现出促凋亡功能* 210
作者：
I. Kuwabara;Yasuko Kuwabara;Riyao Yang;M. Schuler;D. Green;B. Zuraw;D. Hsu;Fu
Galectin-7 is normally expressed in all types of stratified epithelia, but is significantly down-regulated in squamous cell carcinomas. This protein was recently found to be highly inducible by p53 in a colon carcinoma cell line, DLD-1, and designated as PIG1 (for p53-inducedgene 1). We studied transfectants of HeLa and DLD-1 cells ectopically expressing this protein and found that they were more susceptible to apoptosis than control transfectants. This was observed in apoptosis induced by mechanistically distinct stimuli, suggesting that galectin-7 acts on a common point in the apoptosis signaling pathways. Further analyses of actinomycin D-induced apoptosis demonstrated that galectin-7 expression causes enhanced caspase-3 activity and poly(ADP-ribose) polymerase cleavage, and the potentiation of apoptosis by galectin-7 was completely abrogated by a caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone. In addition, galectin-7 transfectants displayed accelerated mitochondrial cytochrome c release and up-regulated JNK activity upon apoptosis induction. Several lines of evidence indicate that the effect on apoptosis is not due to the lectin functioning extracellularly through interactions with cell surface glycoconjugates. In fact, this lectin is found to localize in nuclei and cytoplasm of the transfectants and the transformed keratinocyte line HaCaT. Therefore, galectin-7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. DNA microarray analysis revealed genes that are differentially expressed between galectin-7 and control transfectants. Some of them are potentially contributory to this lectin's proapoptotic function and these include redox-related genes monoamine oxidase B, ryanodine receptor 2, and glutathione S-transferase Mu 3.
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影响因子：
4.4
作者：
I. Kuwabara;Fu-Tong Liu
通讯作者：
I. Kuwabara;Fu-Tong Liu
影响因子：
11.2
作者：
Hidenori Inohara;Shiro Akahani;K. Koths;A. Raz
通讯作者：
Hidenori Inohara;Shiro Akahani;K. Koths;A. Raz
影响因子：
56.9
作者：
HAN, J;LEE, JD;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
4.4
作者：
L. Frigeri;Fuan Liu
通讯作者：
L. Frigeri;Fuan Liu
运动障碍的实验进展：提议的自由基机制的更新。
影响因子：
4.8
作者：
Przedborski,S;Jackson-Lewis,V
通讯作者：
Jackson-Lewis,V