Tibial Cartilage Damage and Deformation at Peak Displacement Compression during Simulated Landing Impact
Tibial Cartilage Damage and Deformation at Peak Displacement Compression during Simulated Landing Impact
复制标题
模拟着陆冲击过程中峰值位移压缩时的胫骨软骨损伤和变形
DOI：
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发表时间：
2010
影响因子：
4.8
通讯作者：
J. Goh
中科院分区：
文献类型：
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作者：
C. Yeow;Yi Hao Ng;P. Lee;J. Goh
背景在着陆冲击过程中，关节软骨在峰值位移压缩点的结构变化是未知的。假设损伤和变形的程度是显着不同的浅，中，深软骨区在峰值位移压缩在模拟着陆冲击与冲击后相比。研究设计对照实验室研究。方法从猪胫骨软骨中提取组织块，分为3种试验条件：非冲击对照、冲击释放和冲击保持。基于单个10 Hz半正矢施加峰值变形为2 mm的冲击压缩以模拟着陆冲击。对于撞击和释放，在撞击后移除负载时，对外植体进行福尔马林固定。对于冲击和保持，立即将外植体浸入福尔马林中，峰值变形保持在2 mm。固定后，对外植体进行组织学检查，获得Mankin评分和软骨厚度。结果在冲击压缩过程中，观察到9.8 ~ 28.1MPa的峰值应力。对于冲击和释放，观察到大量软骨缺损，如表面磨损和裂缝。对于撞击和保持，外植体表现出不太严重的基质损伤，如表面不规则和潮标破坏。Mankin评分在撞击和保持中比在撞击和释放条件中低（指示较少的损伤; P <0.05）。与非冲击对照组相比，在冲击和保持以及冲击和释放条件下，浅表软骨区厚度均降低（P <0.05）。结论冲击压缩的加载阶段不仅对软骨造成实质性损伤和变形，而且卸载阶段通过加剧表面损伤的裂隙扩展而导致整体软骨损伤。临床相关性临床损伤的成像可能会低估损伤期间发生的软骨压缩的程度。软骨组织工程支架的设计必须能够科普加载和卸载阶段的影响，特别是在表浅区，以便修复部位能够像邻近的天然软骨一样发挥功能。
Background Structural changes of articular cartilage at the point of peak displacement compression during a landing impact are unknown. Hypothesis Extent of damage and deformation is significantly different for superficial, middle, and deep cartilage zones at peak displacement compression during simulated landing impact compared with after impact. Study Design Controlled laboratory study. Methods Explants were extracted from porcine tibial cartilages and divided into 3 test conditions: nonimpact control, impact and release, and impact and hold. Impact compression, with peak deformation of 2 mm, was applied based on a single 10-Hz haversine to simulate landing impact. For impact and release, explants were subjected to formalin fixation on removal of load after impact. For impact and hold, explants were immediately immersed in formalin with peak deformation maintained at 2 mm. After fixation, the explants underwent histology, whereby Mankin scores and cartilage thicknesses were obtained. Results Peak stresses of 9.8 to 28.1 MPa were noted during impact compression. For impact and release, substantial cartilage defects such as surface fraying and fissures were observed. For impact and hold, explants exhibited less severe matrix damage, such as superficial irregularities and tidemark disruption. Mankin scores were lower (indicating less damage; P < .05) in impact and hold than in impact and release condition. Superficial cartilage zone thickness was reduced (P < .05) in both impact and hold and impact and release conditions, relative to nonimpact control. Conclusion Not only does the loading phase of impact compression play a role in introducing substantial damage and deformation to cartilage, the unloading phase contributes to overall cartilage damage by exacerbating fissure propagation from surface lesions. Clinical Relevance Imaging of clinical injuries may underestimate the magnitude of cartilage compression that occurred during injury. Cartilage tissue engineering scaffolds must be designed to cope with the effects of loading and unloading phases, especially at the superficial zone, so that the repair site can function as well as does the neighboring native cartilage.
牛关节软骨的损伤性机械挤压诱导软骨细胞凋亡
影响因子：
3.9
作者：
Loening, AM;James, IE;Lark, MW
通讯作者：
Lark, MW
影响因子：
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作者：
Aurich, M;Squires, GR;Cole, AA
通讯作者：
Cole, AA
影响因子：
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作者：
Otsuki, Shuhei;Brinson, Diana C.;Lotz, Martin
通讯作者：
Lotz, Martin