Protein N-glycosylation in the early stage of Spiroplasma eriocheiris infect Eriocheir sinensis
Protein N-glycosylation in the early stage of Spiroplasma eriocheiris infect Eriocheir sinensis
复制标题
绒毛螺原体感染中华绒螯蟹早期蛋白质N-糖基化
DOI：
10.1016/j.aquaculture.2019.734793
复制
发表时间：
2020-02
期刊：
影响因子：
4.5
通讯作者：
Qingguo Meng
中科院分区：
文献类型：
--
作者：
Libo Hou;Yuye Yan;Tao Xiang;Yubo Ma;Wei Gu;Wen Wang;Qingguo Meng
中华绒螯蟹螺旋体（Spiroplasmaeriocheiris）是引起中华绒螯蟹（Eriocheir sinensis）疾病的一种新的致死性病原体，给养殖业造成了灾难性的经济损失。E. sinensis是S.绒螯蟹本研究首次对大肠杆菌N-糖蛋白质组的动态进行了研究。sinensisemocytes under S.绒螯蟹在噬菌体早期感染。本研究对E. sinensisemocytes after S.使用串联质量标签（TMT）标记和亲和富集，然后通过高分辨率液相色谱耦合串联质谱（LC-MS/MS）分析获得绒螯蟹属感染。使用1.2倍的表达变化作为生理上显著的基准，167个差异表达的N-糖基化蛋白被可靠地定量，包括79个上调的糖基化蛋白和88个下调的糖基化蛋白。绒螯菌感染通过基因本体论（GO）注释、蛋白质结构域注释、京都基因与基因组百科全书（KEGG）注释、亚细胞定位注释和蛋白质相互作用网络等方法对差异表达蛋白质进行分析，结果表明，在绒螯镰刀菌感染宿主细胞过程中，多种生物学过程和途径参与其中，如吞噬作用、ECM-受体相互作用、溶酶体、酚氧化酶原系统、通过qRT-PCR技术，从RNA水平上研究了6个糖基化蛋白的转录情况。本研究为进一步了解E.中国对虾及其病原菌。绒螯蟹的蛋白质N-糖基化研究，也为其他甲壳动物蛋白质N-糖基化研究提供参考。
As a novel lethal pathogen ofEriocheir sinensistremor disease,Spiroplasmaeriocheiris, has led into catastrophic economic losses in aquaculture. The hemocytes ofE. sinensisis the first target cells ofS. eriocheiris. Our study is the first time designed to understanding of the N-glycoproteome dynamics ofE. sinensishemocytes underS. eriocheirisinfected at the early phage. In the current study, the N-glycoproteome changes ofE. sinensishemocytes afterS. eriocheirisinfection were obtained using tandem mass tags (TMT) labeling and affinity enrichment followed by high-resolution Liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) analysis. Using a 1.2-fold change in expression as a physiologically significant benchmark, 167 differentially expressed N-glycosylated proteins were reliably quantified, including 79 up-regulated glycosylated proteins and 88 down-regulated glycosylated proteins subsequented toS. eriocheirisinfection. Gene ontology (GO) annotation, protein domain annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, subcellular localization annotation and protein-protein interaction network were used to analyze those significantly differently expression proteins shown that many biological process and pathway are participate inS. eriocheirisinfected host cell, such as phagocytosis, ECM-receptor interaction, lysosome, prophenoloxidase system, and so on. Six selected different expressed glycosylated proteins were studied the transcription at RNA level by qRT-PCR. Our study could serve as a basis to understand the relationship betweenE. sinensisand the pathogenS. eriocheiris, and also provide reference to study protein N-glycosylation in other crustaceans.
登录
查看更多内容
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
T. Braulke;A. Raas‐Rothschild;S. Kornfeld
通讯作者：
T. Braulke;A. Raas‐Rothschild;S. Kornfeld
影响因子：
2.9
作者：
T. Xing;A. Laroche
通讯作者：
T. Xing;A. Laroche
影响因子：
4.7
作者：
Lili Shi;S. Chan;Chaozheng Li;Shuang Zhang
通讯作者：
Lili Shi;S. Chan;Chaozheng Li;Shuang Zhang
影响因子：
2.6
作者：
Wen Wang;Jianxiu Chen;K. Du;Zaikuan Xu
通讯作者：
Wen Wang;Jianxiu Chen;K. Du;Zaikuan Xu
影响因子：
3.2
作者：
W. Weisburg;J. G. Tully;D. Rose;J P Petzel;H. Oyaizu;D. Yang;L. Mandelco;J. Sechrest;T G Lawr
通讯作者：
W. Weisburg;J. G. Tully;D. Rose;J P Petzel;H. Oyaizu;D. Yang;L. Mandelco;J. Sechrest;T G Lawr