High reproducibility of adhesion formation in rat with meso-stitch approximation of injured cecum and abdominal wall.
High reproducibility of adhesion formation in rat with meso-stitch approximation of injured cecum and abdominal wall.
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DOI：
10.7150/ijms.8870
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发表时间：
2015
影响因子：
3.6
通讯作者：
Winny M
中科院分区：
文献类型：
--
作者：
Poehnert D;Abbas M;Kreipe HH;Klempnauer J;Winny M
目的：手术后腹膜粘连是一种常见的、严重的病理，伴有严重的并发症。适当的动物粘连模型对于评估防粘连医疗器械至关重要。本研究介绍了一种已建立的大鼠模型的变体，在该模型中，以优异的再现性诱导了最高程度的粘连（OPAM =优化的腹膜粘连模型）。因此，该模型似乎有资格研究防粘连装置的效果。方法：24只刘易斯雄性大鼠随机分为OPAM组、WSFX组、假OPAM组、假WSFX组。OPAM技术包括盲肠磨损、创建腹壁缺损以及通过缝线对合损伤区域，并与无缝线固定的病变设置（WSFX）进行了比较。在第7天处死所有大鼠。进行了肉眼和组织病理学评价。使用方差分析和邓尼特检验对结果进行统计分析。结果如下：在OPAM大鼠中，肉眼分析显示盲肠与腹壁粘连的发生率为90%，所有粘连均表现为完全凝集。在WSFX动物中，粘连形成的发生率为75%，而在两个假手术组中根本没有粘连。组织学检查均见结肠平滑肌与腹壁骨骼肌粘连。结论：OPAM技术提供了比传统方法更好的受伤部位粘连的可能性。所有OPAM粘连均为最高程度粘连，即凝集。由于粘连形成的发生率和范围具有较高的重现性，建议将OPAM用于防粘连医疗器械的试验。
Objective: Peritoneal adhesions following surgery are a common, serious pathology with severe complications. Appropriate animal adhesion models are essential for the assessment of adhesion preventing medical devices. This study introduces a variation of an established rat model in which highest degree adhesions are induced with excellent reproducibility (OPAM = optimized peritoneal adhesion model). Thus, this model seems to be eligible to study effects of adhesion preventing devices. Methods: 24 Lewis male rats were divided into four groups (OPAM, WSFX, sham-OPAM, sham-WSFX). The OPAM technique comprised cecal abrasion, creation of an abdominal wall defect plus approximation of injured areas by a suture, which was compared to a setting of lesions without suture fixation (WSFX). All rats were sacrificed at day 7. Macroscopic and histopathological evaluations were performed. Results were statistically analyzed using ANOVA and Dunnett's test. Results: In OPAM rats macroscopic analyses revealed a 90% incidence adhesion of cecum to the abdominal wall, all adhesions imposing as complete agglutination. In WSFX animals incidence of adhesions formation was 75%, while in both sham groups there were no adhesions at all. Histology showed the structure of adhesions with merged smooth muscle of colon and skeletal muscle of abdominal wall in all cases. Conclusion: OPAM technique provides adhesions of injured areas with a better probability than with conventional methods. All OPAM adhesions impressed as highest degree adhesions, i.e. agglutination. Due to high reproducibility in incidence and extend of adhesion formation, the OPAM is recommended for testing of adhesion prevention medical devices.
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影响因子：
2.2
作者：
Lucas, PA;Warejcka, DJ;Lee, BY
通讯作者：
Lee, BY
影响因子：
5.2
作者：
Ray, NF;Denton, WG;Perry, S
通讯作者：
Perry, S
影响因子：
3.9
作者：
Oncel, M;Remzi, FH;Fazio, VW
通讯作者：
Fazio, VW
影响因子：
1.6
作者：
Thaler, K;Mack, JA;Abramson, SR
通讯作者：
Abramson, SR
影响因子：
3.3
作者：
Oncel, M;Remzi, F H;Fazio, V W
通讯作者：
Fazio, V W