HLA-G polymorphisms and soluble HLA-G protein levels in women with recurrent pregnancy loss from Basrah province in Iraq.
HLA-G polymorphisms and soluble HLA-G protein levels in women with recurrent pregnancy loss from Basrah province in Iraq.
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DOI：
10.1016/j.humimm.2012.05.009
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发表时间：
2012-08
期刊：
影响因子：
2.7
通讯作者：
Ober, Carole
中科院分区：
文献类型：
--
作者：
Jassem, Raghed M.;Shani, Wafaa Sadoon;Loisel, Dagan A.;Sharief, Maysoon;Billstrand, Christine;Ober, Carole
HLA-G是一种非经典的I类主要组织相容性复合体（MHC）基因，具有免疫调节特性，可能在维持成功妊娠中发挥作用。在这项研究中，我们调查了HLA-G多态性在伊拉克妇女复发性妊娠丢失（RPL）风险和循环可溶性HLA-G水平中的作用。从伊拉克巴士拉省50名RPL妇女和50名健康孕妇在妊娠9至12周收集的血液样本中获得DNA和血浆。通过ELISA检测，与健康对照组相比，RPL病例的sHLA-G水平中位数显著降低（分别为21.4对38.8 U/ml, P = 0.025），并且随着母亲年龄的增加而降低（P = 0.0051）。然而，HLA-G等位基因和单倍型频率在病例和对照组之间没有显著差异（所有测试的P值均≥0.12）。相比之下，三等位基因启动子多态性的C等位基因（CC）的纯合性（- 725C/G/T）与CG或CT基因型的sHLA- G浓度较低相关（中位水平分别为21.1、40.1和42.6 U/ml, P = 0.0089）。这些结果表明，HLA-G基因型影响妊娠期间循环sHLA-G水平，但与RPL风险无显著相关性。
HLA-G is a nonclassical, class I major histocompatibility complex (MHC) gene that exhibits immunomodulatory properties and likely plays a role in the maintenance of successful pregnancy. In this study, we investigated the role of HLA-G polymorphisms on risk for recurrent pregnancy loss (RPL) and on circulating levels of soluble (s)HLA-G in Iraqi women. DNA and plasma were obtained from blood samples collected at 9 to 12 weeks gestation from 50 women with RPL and 50 healthy pregnant women in Basrah province, Iraq. As measured by ELISA, median sHLA-G levels were significantly lower in the RPL cases compared to healthy controls (21.4 vs. 38.8 U/ml, respectively; P = 0.025), and decreased with increasing maternal age (P = 0.0051). However, HLA-G allele and haplotype frequencies did not differ significantly between cases and controls (P values ≥ 0.12 for all tests). In contrast, homozygosity for the C allele (CC) at a tri- allelic promoter polymorphism, −725C/G/T, was associated with lower concentrations of sHLA- G compared to the CG or CT genotypes (median levels 21.1 vs. 40.1 vs. 42.6 U/ml, respectively; P = 0.0089). These results demonstrate that HLA-G genotype influences circulating sHLA-G levels during pregnancy but is not significantly associated with risk of RPL.
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影响因子：
3.2
作者：
Ishitani, A;Kishida, M;Hatake, K
通讯作者：
Hatake, K
影响因子：
3.2
作者：
Hviid, TVF;Hylenius, S;Nielsen, LG
通讯作者：
Nielsen, LG
影响因子：
--
作者：
Hviid, TV;Hylenius, S;Christiansen, OB
通讯作者：
Christiansen, OB
影响因子：
3.4
作者：
Aldrich, C;Verp, MS;Ober, C
通讯作者：
Ober, C
DOI：
10.1111/j.1365-2370.2004.00487.x
发表时间：
2004-12-01
期刊：
EUROPEAN JOURNAL OF IMMUNOGENETICS
影响因子：
--
作者：
Abbas, A;Tripathi, P;Agrawal, S
通讯作者：
Agrawal, S