Immunohistological detection of human herpes virus 6 in formalin‐fixed, paraffin‐embedded lung tissues
Immunohistological detection of human herpes virus 6 in formalin‐fixed, paraffin‐embedded lung tissues
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福尔马林固定、石蜡包埋肺组织中人疱疹病毒 6 型的免疫组织学检测
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发表时间：
1993
影响因子：
12.7
通讯作者：
R. Fitzmaurice
中科院分区：
文献类型：
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作者：
Anil Pitalia;J. Liu;A. Freemont;D. Morris;R. Fitzmaurice
人类疱疹病毒 6 型 (HHV-6) 感染在健康个体中广泛存在。唯一明确的疾病关联是婴儿的急疹，尽管该病毒与多种其他疾病有关，包括骨髓同种异体移植受者的间质性肺炎。为了研究 HHV-6 在后一种疾病中的作用，我们开发了一种优化的染色方法，用于在常规处理的死后组织中展示特定抗原。使用改良的亲和素-生物素复合物 (ABC) 方法，使用抗 HHV-6、巨细胞病毒 (CMV) 和腺病毒的单克隆抗体，对 8 名死于间质性肺炎的免疫功能低下患者的福尔马林固定、石蜡包埋的肺组织进行免疫过氧化物酶染色。在所研究的 8 名患者中，有 6 名获得了 HHV-6 染色，并且存在于肺细胞和巨噬细胞中。分别在 4 名和 6 名患者中鉴定出 CMV 和腺病毒抗原。虽然 6 名患者的肺组织含有不止一种病毒，但没有证据表明单克隆抗体之间存在交叉反应。我们证明，使用单克隆抗体在死后肺组织中准确定位 HHV-6 是可能的，并得出结论，单独使用 HHV-6 或与其他病毒联合使用可能在骨髓移植或化疗后间质性肺炎的发生中发挥作用。
Human herpes virus type 6 (HHV‐6) infection is widespread in healthy individuals. The only definite disease association is with exanthem subitum in infants though the virus has been linked with a variety of other diseases including interstitial pneumonitis in bone marrow allograft recipients. In order to investigate the role of HHV‐6 in the latter disease we have developed an optimised staining method for the demonstration of specific antigen in routinely processed post‐mortem tissues. Formalin‐fixed, paraffin‐embedded lung tissue from 8 immunocompromised patients who died from interstitial pneumonitis was subjected to immunoperoxidase staining with monoclonal antibodies against HHV‐6, cytomegalovirus (CMV), and adenovirus, using a modified avidin‐biotin complex (ABC) method. Staining for HHV‐6 was obtained in 6 of the 8 patients studied and was present in pneumocytes and macrophages. CMV and adenovirus antigens were identified in 4 and 6 patients, respectively. Whilst the lung tissue of 6 patients contained more than one virus, there was no evidence of cross‐reactivity between the monoclonal antibodies. We demonstrated that accurate localisation of HHV‐6 using monoclonal antibodies is possible in post‐mortem lung tissue and conclude that either HHV‐6 alone or in combination with other viruses may play a role in the development of interstitial pneumonitis following bone marrow transplantation or chemotherapy.
从 HIV 感染者唾液中回收的 HHV-6 新毒株 (HHV-6SF) 的特征。
影响因子：
3.7
作者：
Levy,JA;Ferro,F;Lennette,ET;Oshiro,L;Poulin,L
通讯作者：
Poulin,L