Effects of sinomenine on the expression of microRNA-155 in 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice.
Effects of sinomenine on the expression of microRNA-155 in 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice.
复制标题
DOI：
10.1371/journal.pone.0073757
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xia B
中科院分区：
文献类型：
--
作者：
Yu Q;Zhu S;Zhou R;Yi F;Bing Y;Huang S;Wang Z;Wang C;Xia B
青藤碱是一种从青藤根中分离出来的纯中药生物碱，已被证明具有抗炎和免疫抑制作用。 MicroRNA (miRNA) 通过分子效应途径的协调调节逐渐被认为是疾病发病机制的关键介质。通过滴注5%（w/v）2,4,6-三硝基苯磺酸（TNBS）诱导小鼠结肠炎后，口服青藤碱100或200 mg/kg，每天一次，连续7天。我们评估了体重、存活率、腹泻评分、组织学评分和髓过氧化物酶（MPO）活性。分别通过定量RT-PCR和免疫组织化学测定miR-155、c-Maf、TNF-α和IFN-γ的mRNA和蛋白表达水平。与未治疗的小鼠相比，经青藤碱（100或200 mg/kg）治疗的TNBS诱导结肠炎小鼠的体重、存活率、腹泻评分、组织学评分和MPO活性均显着改善。两种剂量的青藤碱均显着降低了 c-Maf、TNF-α 和 IFN-γ 的 mRNA 和蛋白表达水平，而在 TNBS 诱导的结肠炎中，这些水平升高。此外，与未经治疗的TNBS诱导的小鼠结肠炎相比，200 mg/kg剂量的青藤碱可显着降低miR-155表达水平71%（p = 0.025）。我们的研究评估了青藤碱通过 miRNA-155 对 TNBS 诱导的结肠炎小鼠的抗炎反应的作用和潜在机制。我们的研究结果表明，青藤碱通过下调 miR-155 和几种相关炎症细胞因子的水平，对 TNBS 诱导的结肠炎具有抗炎作用。
Sinomenine, a pure alkaloid isolated in Chinese medicine from the root of Sinomenium acutum, has been demonstrated to have anti-inflammatory and immunosuppressive effects. MicroRNAs (miRNAs) are gradually being recognized as critical mediators of disease pathogenesis via coordinated regulation of molecular effector pathways. After colitis was induced in mice by instillation of 5% (w/v) 2,4,6-trinitrobenzenesulfonic acid (TNBS), sinomenine at a dose of 100 or 200 mg/kg was orally administered once daily for 7 days. We evaluated body weight, survival rate, diarrhea score, histological score and myeloperoxidase (MPO) activity. The mRNA and protein expression levels of miR-155, c-Maf, TNF-α and IFN-γ were determined by quantitative RT-PCR and immunohistochemistry, respectively. Sinomenine (100 or 200 mg/kg)-treated mice with TNBS-induced colitis were significantly improved in terms of body weight, survival rate, diarrhea score, histological score and MPO activity compared with untreated mice. Both dosages of sinomenine significantly decreased the mRNA and protein expression levels of c-Maf, TNF-α and IFN-γ, which elevated in TNBS-induced colitis. Furthermore, sinomenine at a dose of 200 mg/kg significantly decreased the level of miR-155 expression by 71% (p = 0.025) compared with untreated TNBS-induced colitis in mice. Our study evaluated the effects and potential mechanisms of sinomenine in the anti-inflammatory response via miRNA-155 in mice with TNBS-induced colitis. Our findings suggest that sinomenine has anti-inflammatory effects on TNBS-induced colitis by down-regulating the levels of miR-155 and several related inflammatory cytokines.
登录
查看更多内容
影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1126/science.1139253
发表时间：
2007-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodriguez A;Vigorito E;Clare S;Warren MV;Couttet P;Soond DR;van Dongen S;Grocock RJ;Das PP;Miska EA;Vetrie D;Okkenhaug K;Enright AJ;Dougan G;Turner M;Bradley A
通讯作者：
Bradley A
影响因子：
29.4
作者：
MORRIS, GP;BECK, PL;WALLACE, JL
通讯作者：
WALLACE, JL
影响因子：
6.2
作者：
Mark, W;Schneeberger, S;Margreiter, R
通讯作者：
Margreiter, R
影响因子：
2.7
作者：
Shi, R;Chiang, VL
通讯作者：
Chiang, VL