Body temperature (37 C) specifically down-regulates the messenger ribonucleic acid for the major sperm surface antigen CD52 in epididymal cell culture.
Body temperature (37 C) specifically down-regulates the messenger ribonucleic acid for the major sperm surface antigen CD52 in epididymal cell culture.
复制标题
体温（37°C）特异性下调附睾细胞培养物中主要精子表面抗原 CD52 的信使核糖核酸。
作者：
I. Pera;R. Ivell;C. Kirchhoff
为了研究阴囊温度与体温在附睾功能中的作用，我们建立了一个简化的狗附睾细胞培养系统，其中细胞显示出与人附睾相似的基因表达模式，并保留了附睾上皮细胞的许多特征。培养的细胞具有上皮型细胞骨架、核雄激素受体蛋白和显著的温度反应性。与33 C相比，将细胞暴露于37 C的培养温度对丰富的附睾信使RNA（mRNA）CE 5的水平具有快速且不可逆的抑制作用，CE 5代表人CD 52/HE 5 mRNA的犬对应物，编码主要的糖基磷酸肌醇（GPI）锚定精子膜糖肽。温度对mRNA的影响是直接和特异的，不通过温度对睾丸的影响介导，也不影响其他附睾mRNA。将细胞暴露于5，6-二氯-1-β-D-呋喃核糖基苯并咪唑（25微克/ml培养基）和放线菌酮（2微克/ml）表明，CD 52/CE 5 mRNA的稳态水平可能是通过改变这种特定mRNA对细胞外温度刺激的半衰期而在转录后控制的。
To study the role of scrotal vs. body temperature in epididymal function we established a simplified cell culture system from the dog epididymis in which the cells showed a pattern of gene expression similar to that in the human epididymis and retained many characteristics of epididymal epithelial cells. The cultured cells had an epithelial-type cytoskeleton, nuclear androgen receptor protein, and a striking temperature responsiveness. Exposure of the cells to a culture temperature of 37 C, compared to 33 C, had a fast and irreversibly suppressive effect on the levels of an abundant epididymal messenger RNA (mRNA), CE5, which represents the canine counterpart of the human CD52/HE5 mRNA, encoding a major glycosylphoshatidylinnositol (GPI)-anchored sperm membrane glycopeptide. The temperature effect on the mRNA was a direct and specific one, not mediated by temperature influences on the testis and not affecting other epididymal mRNAs. Exposure of the cells to 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (25 micrograms/ml culture medium) and cycloheximide (2 micrograms/ml) suggested that the steady state levels of CD52/CE5 mRNA may be controlled posttranscriptionally by changing the half-life of this specific mRNA in response to an extracellular temperature stimulus.
影响因子：
4.8
作者：
PRINS, GS;BIRCH, L;GREENE, GL
通讯作者：
GREENE, GL
放线菌酮对 rRNA、5S RNA 和 tRNA 基因转录的影响。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gokal,PK;Cavanaugh,AH;ThompsonJr,EA
通讯作者：
ThompsonJr,EA